CRYGD sirna

CRYGD siRNA (Human)

Cat. Num. AAA8204909

• Gene Names: CRYGD; CCP; PCC; CACA; CCA3; CRYG4; CTRCT4; cry-g-D
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CRYGD; CRYGD siRNA (Human); CRYG4; Gamma-crystallin D; Gamma-D-crystallin; Gamma-crystallin 4; CRYGD sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CRYGD siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 174

• Applicable Applications for CRYGD sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CRYGD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CRYGD sirna

siRNA to inhibit CRYGD expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13377002
• NCBI GeneID: 1421
• NCBI Accession #: NP_008822.2
• NCBI GenBank Nucleotide #: NM_006891.3
• UniProt Accession #: P07320; Q17RF7; Q53R51; Q99681
• Molecular Weight: 20,738 Da
• NCBI Official Full Name: gamma-crystallin D
• NCBI Official Synonym Full Names: crystallin, gamma D
• NCBI Official Symbol: CRYGD
• NCBI Official Synonym Symbols: CCP; PCC; CACA; CCA3; CRYG4; CTRCT4; cry-g-D
• NCBI Protein Information: gamma-crystallin D
• UniProt Protein Name: Gamma-crystallin D
• Protein Family: Gamma-crystallin
• UniProt Gene Name: CRYGD
• UniProt Synonym Gene Names: CRYG4
• UniProt Entry Name: CRGD_HUMAN

NCBI Description

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. [provided by RefSeq, Jul 2008]

Uniprot Description

CRYGD: a major structural protein of the eye lens. A member of the beta/gamma-crystallin family. Defects are the cause of congenital zonular cataract with sutural opacities. Two splice-variant isoforms have been described.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 2q33.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; structural constituent of eye lens

Biological Process: lens development in camera-type eye; visual perception

Disease: Cataract 4, Multiple Types