COL25A1 sirna

COL25A1 siRNA (Human)

Cat. Num. AAA8223389

• Gene Names: COL25A1; AMY; CLAC; CLACP; CFEOM5; CLAC-P
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: COL25A1; COL25A1 siRNA (Human); Collagen alpha-1(XXV) chain; Alzheimer disease amyloid-associated protein; AMY; CLAC-P; COL25A1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: COL25A1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 642

• Applicable Applications for COL25A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human COL25A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for COL25A1 sirna

siRNA to inhibit COL25A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 38570073
• NCBI GeneID: 84570
• NCBI Accession #: NP_115907.2
• NCBI GenBank Nucleotide #: NM_032518.2
• UniProt Accession #: Q9BXS0; Q9BXR9; A8MPZ6
• Molecular Weight: 57,619 Da
• NCBI Official Full Name: collagen alpha-1(XXV) chain isoform 2
• NCBI Official Synonym Full Names: collagen, type XXV, alpha 1
• NCBI Official Symbol: COL25A1
• NCBI Official Synonym Symbols: AMY; CLAC; CLACP; CFEOM5; CLAC-P
• NCBI Protein Information: collagen alpha-1(XXV) chain
• UniProt Protein Name: Collagen alpha-1(XXV) chain
• UniProt Gene Name: COL25A1
• UniProt Synonym Gene Names: AMY; CLAC
• UniProt Entry Name: COPA1_HUMAN

NCBI Description

This gene encodes a brain-specific membrane associated collagen. A product of proteolytic processing of the encoded protein, CLAC (collagenous Alzheimer amyloid plaque component), binds to amyloid beta-peptides found in Alzheimer amyloid plaques but CLAC inhibits rather than facilitates amyloid fibril elongation (PMID: 16300410). A study of over-expression of this collagen in mice, however, found changes in pathology and behavior suggesting that the encoded protein may promote amyloid plaque formation (PMID: 19548013). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

COL25A1: Inhibits fibrillization of beta amyloid peptide during the elongation phase. Has also been shown to assemble amyloid fibrils into protease-resistant aggregates. Binds heparin. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: extracellular space; collagen; integral to plasma membrane; endoplasmic reticulum lumen; integral to membrane; plasma membrane; extracellular region

Molecular Function: heparin binding; beta-amyloid binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis

Disease: Fibrosis Of Extraocular Muscles, Congenital, 5