COL18A1 sirna

COL18A1 siRNA (Human)

Cat. Num. AAA8226743

• Gene Names: COL18A1; KS; KNO; KNO1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: COL18A1; COL18A1 siRNA (Human); Collagen alpha-1(XVIII) chain; COL18A1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: COL18A1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,339

• Applicable Applications for COL18A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human COL18A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for COL18A1 sirna

siRNA to inhibit COL18A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 110611235
• NCBI GeneID: 80781
• NCBI Accession #: NP_085059.2
• NCBI GenBank Nucleotide #: NM_030582.3
• UniProt Accession #: P39060; Q58EX6; Q6RZ39; Q6RZ40; Q6RZ41; Q8N4S4; Q8WXI5; Q96T70; Q9UK38; Q9Y6Q7; Q9Y6Q8; A8MVI4
• Molecular Weight: 135,761 Da
• NCBI Official Full Name: collagen alpha-1(XVIII) chain isoform 1 preproprotein
• NCBI Official Synonym Full Names: collagen, type XVIII, alpha 1
• NCBI Official Symbol: COL18A1
• NCBI Official Synonym Symbols: KS; KNO; KNO1
• NCBI Protein Information: collagen alpha-1(XVIII) chain
• UniProt Protein Name: Collagen alpha-1(XVIII) chain
• Protein Family: Collagen
• UniProt Gene Name: COL18A1
• UniProt Entry Name: COIA1_HUMAN

NCBI Description

This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

COL18A1: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Defects in COL18A1 are a cause of Knobloch syndrome type 1 (KNO1). An autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. Belongs to the multiplexin collagen family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular matrix; extracellular space; collagen; endoplasmic reticulum lumen; extracellular region; basement membrane

Molecular Function: identical protein binding; protein binding; metal ion binding; structural molecule activity

Biological Process: response to drug; extracellular matrix disassembly; collagen catabolic process; negative regulation of cell proliferation; organ morphogenesis; extracellular matrix organization and biogenesis; visual perception; positive regulation of cell proliferation; angiogenesis; endothelial cell morphogenesis; cell adhesion; response to hydrostatic pressure; positive regulation of cell migration

Disease: Knobloch Syndrome 1