COL17A1 sirna

COL17A1 siRNA (Human)

Cat. Num. AAA8204334

• Gene Names: COL17A1; BP180; BPA-2; BPAG2; LAD-1; BA16H23.2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: COL17A1; COL17A1 siRNA (Human); BP180; BPAG2; Collagen alpha-1(XVII) chain; 180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2; COL17A1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: COL17A1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1497

• Applicable Applications for COL17A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human COL17A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for COL17A1 sirna

siRNA to inhibit COL17A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 119829187
• NCBI GeneID: 1308
• NCBI Accession #: NP_000485.3
• NCBI GenBank Nucleotide #: NM_000494.3
• UniProt Accession #: Q9UMD9; Q02802; Q5JV36; Q99018; Q9NQK9; Q9UC14
• Molecular Weight: 142,344 Da
• NCBI Official Full Name: collagen alpha-1(XVII) chain
• NCBI Official Synonym Full Names: collagen, type XVII, alpha 1
• NCBI Official Symbol: COL17A1
• NCBI Official Synonym Symbols: BP180; BPA-2; BPAG2; LAD-1; BA16H23.2
• NCBI Protein Information: collagen alpha-1(XVII) chain
• UniProt Protein Name: Collagen alpha-1(XVII) chain
• UniProt Gene Name: COL17A1
• UniProt Synonym Gene Names: BP180; BPAG2; LAD-1; 97 kDa LAD antigen; 97-LAD; LABD97
• UniProt Entry Name: COHA1_HUMAN

NCBI Description

This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008]

Uniprot Description

COL17A1: May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane. Defects in COL17A1 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). GABEB is a non- lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Extracellular matrix; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q24.3

Cellular Component: collagen; hemidesmosome; integral to plasma membrane; endoplasmic reticulum lumen; extracellular region; plasma membrane; basement membrane; intercellular junction

Molecular Function: protein binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; epidermis development; hemidesmosome assembly; cell-matrix adhesion

Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type