COL11A2 sirna

COL11A2 siRNA (Human)

Cat. Num. AAA8240433

• Gene Names: COL11A2; HKE5; PARP; STL3; FBCG2; DFNA13; DFNB53
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: COL11A2; COL11A2 siRNA (Human); Collagen alpha-2(XI) chain; COL11A2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: COL11A2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 290

• Applicable Applications for COL11A2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human COL11A2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for COL11A2 sirna

siRNA to inhibit COL11A2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 254939712
• NCBI GeneID: 1302
• NCBI Accession #: NP_001157243.1
• NCBI GenBank Nucleotide #: NM_001163771.1
• UniProt Accession #: P13942; Q07751; Q13271; Q13272; Q13273; Q5JP94; Q5SUI8; Q7Z6C3; Q99866; Q9UIP9; A6NLX2; E7ER90
• Molecular Weight: 31,960 Da
• NCBI Official Full Name: collagen alpha-2(XI) chain isoform 4
• NCBI Official Synonym Full Names: collagen, type XI, alpha 2
• NCBI Official Symbol: COL11A2
• NCBI Official Synonym Symbols: HKE5; PARP; STL3; FBCG2; DFNA13; DFNB53
• NCBI Protein Information: collagen alpha-2(XI) chain
• UniProt Protein Name: Collagen alpha-2(XI) chain
• Protein Family: Collagen
• UniProt Gene Name: COL11A2
• UniProt Entry Name: COBA2_HUMAN

NCBI Description

This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. It is located on chromosome 6 very close to but separate from the gene for retinoid X receptor beta. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Proteolytic processing of this type XI chain produces PARP, a proline/arginine-rich protein that is an amino terminal domain. Mutations in this gene are associated with type III Stickler syndrome, otospondylomegaepiphyseal dysplasia (OSMED syndrome), Weissenbacher-Zweymuller syndrome, autosomal dominant non-syndromic sensorineural type 13 deafness (DFNA13), and autosomal recessive non-syndromic sensorineural type 53 deafness (DFNB53). Alternative splicing results in multiple transcript variants. A related pseudogene is located nearby on chromosome 6. [provided by RefSeq, Jul 2009]

Uniprot Description

COL11A2: May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils. Defects in COL11A2 are the cause of Stickler syndrome type 3 (STL3). STL3 is an autosomal dominant non- ocular form of Stickler syndrome. Classical Stickler syndrome associates ocular signs with more or less complete forms of Pierre Robin sequence, bone disorders and sensorineural deafness. Ocular symptoms are absent in STL3. Robin sequence includes an opening in the roof of the mouth (a cleft palate), a large tongue (macroglossia), and a small lower jaw (micrognathia). Bones are affected by slight platyspondylisis and large, often defective epiphyses. Juvenile joint laxity is followed by early signs of arthrosis. The degree of hearing loss varies among affected individuals and may become more severe over time. Syndrome expressivity is variable. Defects in COL11A2 are the cause of autosomal recessive otospondylomegaepiphyseal dysplasia (OSMED). OSMED is a skeletal dysplasia accompanied by severe hearing loss. The phenotype overlaps that of autosomal dominant skeletal disorders (Stickler and Marshall syndromes) but can be distinguished by disproportionately short limbs and lack of ocular involvement. Defects in COL11A2 are the cause of Weissenbacher- Zweymueller syndrome (WZS). WZS is an autosomal dominant disorder allelic with STL3 and OSMED. WZS is also referred to as heterozygous OSMED. Defects in COL11A2 are the cause of deafness autosomal dominant type 13 (DFNA13). DFNA13 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Defects in COL11A2 are the cause of deafness autosomal recessive type 53 (DFNB53). Defects in COL11A2 are the cause of fibrochondrogenesis type 2 (FBCG2). A severe skeletal dysplasia characterized by a flat midface, short long bones, short ribs with broad metaphyses, and vertebral bodies that show distinctive hypoplastic posterior ends and rounded anterior ends, giving the vertebral bodies a pinched appearance on lateral radiographic views. The chest is small, causing perinatal respiratory problems which usually, but not always, result in lethality. Affected individuals who survive the neonatal period have high myopia, mild to moderate hearing loss, and severe skeletal dysplasia. Belongs to the fibrillar collagen family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: collagen type XI; endoplasmic reticulum lumen; extracellular region

Molecular Function: protein binding, bridging; protein binding; metal ion binding; extracellular matrix structural constituent conferring tensile strength

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; collagen fibril organization; sensory perception of sound; cartilage development; palate development; soft palate development; skeletal development

Disease: Deafness, Autosomal Dominant 13; Weissenbacher-zweymuller Syndrome; Otospondylomegaepiphyseal Dysplasia; Deafness, Autosomal Recessive 53; Fibrochondrogenesis 2; Stickler Syndrome, Type Iii