CLIC5 sirna

CLIC5 siRNA (Human)

Cat. Num. AAA8211867

• Gene Names: CLIC5; MST130; DFNB102; DFNB103; MSTP130
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CLIC5; CLIC5 siRNA (Human); Chloride intracellular channel protein 5; CLIC5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CLIC5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 410

• Applicable Applications for CLIC5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CLIC5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CLIC5 sirna

siRNA to inhibit CLIC5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 166197662
• NCBI GeneID: 53405
• NCBI Accession #: NP_001107558.1
• NCBI GenBank Nucleotide #: NM_001114086.1
• UniProt Accession #: Q9NZA1; Q5T4Z0; Q8NBY3; Q96JT5; Q9BWZ0; B3KUF1
• Molecular Weight: 22,830 Da
• NCBI Official Full Name: chloride intracellular channel protein 5 isoform a
• NCBI Official Synonym Full Names: chloride intracellular channel 5
• NCBI Official Symbol: CLIC5
• NCBI Official Synonym Symbols: MST130; DFNB102; DFNB103; MSTP130
• NCBI Protein Information: chloride intracellular channel protein 5
• UniProt Protein Name: Chloride intracellular channel protein 5
• Protein Family: Chloride intracellular channel protein
• UniProt Gene Name: CLIC5
• UniProt Entry Name: CLIC5_HUMAN

NCBI Description

This gene encodes a member of the chloride intracellular channel (CLIC) family of chloride ion channels. The encoded protein associates with actin-based cytoskeletal structures and may play a role in multiple processes including hair cell stereocilia formation, myoblast proliferation and glomerular podocyte and endothelial cell maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

CLIC5: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Required for normal formation of stereocilia in the inner ear and normal development of the organ of Corti. Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture. Belongs to the chloride channel CLIC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: Golgi apparatus; stereocilium; microtubule organizing center; cell cortex; actin cytoskeleton

Molecular Function: chloride channel activity; protein binding; voltage-gated ion channel activity

Biological Process: diet induced thermogenesis; protein localization; sensory perception of sound; transport; chloride transport; auditory receptor cell stereocilium organization and biogenesis; female pregnancy; neuromuscular process controlling balance

Disease: Deafness, Autosomal Recessive 103