CLCA1 sirna

CLCA1 siRNA (Human)

Cat. Num. AAA8206635

• Gene Names: CLCA1; CACC; GOB5; CACC1; CLCRG1; CaCC-1; hCLCA1; hCaCC-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CLCA1; CLCA1 siRNA (Human); CACC1; Calcium-activated chloride channel regulator 1; Calcium-activated chloride channel family member 1; hCLCA1; Calcium-activated chloride channel protein 1; CaCC-1; hCaCC-1; CLCA1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CLCA1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 914

• Applicable Applications for CLCA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CLCA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CLCA1 sirna

siRNA to inhibit CLCA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 110611231
• NCBI GeneID: 1179
• NCBI Accession #: NP_001276.2
• NCBI GenBank Nucleotide #: NM_001285.3
• UniProt Accession #: A8K7I4; O95151; Q5TDF4; Q9UNF6; Q9UPC6; B2RAV5
• Molecular Weight: 100,226 Da
• NCBI Official Full Name: calcium-activated chloride channel regulator 1
• NCBI Official Synonym Full Names: chloride channel accessory 1
• NCBI Official Symbol: CLCA1
• NCBI Official Synonym Symbols: CACC; GOB5; CACC1; CLCRG1; CaCC-1; hCLCA1; hCaCC-1
• NCBI Protein Information: calcium-activated chloride channel regulator 1
• UniProt Protein Name: Calcium-activated chloride channel regulator 1
• Protein Family: Calcium-activated chloride channel regulator
• UniProt Gene Name: CLCA1
• UniProt Synonym Gene Names: CACC1; hCLCA1; CaCC-1; hCaCC-1
• UniProt Entry Name: CLCA1_HUMAN

NCBI Description

This gene encodes a member of the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same region on chromosome 1p31-p22 and share a high degree of homology in size, sequence, and predicted structure, but differ significantly in their tissue distributions. The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although the site at which the precursor is cleaved has not been precisely determined. The encoded protein may be involved in mediating calcium-activated chloride conductance in the intestine. [provided by RefSeq, Jul 2008]

Uniprot Description

CLCA1: May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC. Belongs to the CLCR family.

Protein type: Channel, chloride; Vesicle

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: extracellular space; zymogen granule membrane; microvillus; integral to plasma membrane; plasma membrane

Molecular Function: chloride channel activity; metallopeptidase activity; metal ion binding

Biological Process: transport; calcium ion transport; chloride transport; proteolysis; transmembrane transport