CERS1 sirna

CERS1 siRNA (Human)

Cat. Num. AAA8202815

• Gene Names: CERS1; EPM8; LAG1; UOG1; LASS1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CERS1; CERS1 siRNA (Human); LAG1; LASS1; UOG1; Ceramide synthase 1; CerS1; LAG1 longevity assurance homolog 1; Longevity assurance gene 1 protein homolog 1; Protein UOG-1; CERS1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CERS1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 350

• Applicable Applications for CERS1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CERS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CERS1 sirna

siRNA to inhibit CERS1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11641421
• NCBI GeneID: 10715
• NCBI Accession #: NP_067090.1
• NCBI GenBank Nucleotide #: NM_021267.4
• UniProt Accession #: P27544
• Molecular Weight: 37,983 Da
• NCBI Official Full Name: ceramide synthase 1 isoform 1
• NCBI Official Synonym Full Names: ceramide synthase 1
• NCBI Official Symbol: CERS1
• NCBI Official Synonym Symbols: EPM8; LAG1; UOG1; LASS1
• NCBI Protein Information: ceramide synthase 1
• UniProt Protein Name: Ceramide synthase 1
• Protein Family: Ceramide synthase
• UniProt Gene Name: CERS1
• UniProt Synonym Gene Names: LAG1; LASS1; UOG1; CerS1
• UniProt Entry Name: CERS1_HUMAN

NCBI Description

This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site that is cleaved to produce a mature protein containing seven conserved cysteine residues. Members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in yeast suggest that the encoded protein is involved in aging. This protein is transcribed from a monocistronic mRNA as well as a bicistronic mRNA, which also encodes growth differentiation factor 1. [provided by RefSeq, Jul 2008]

Uniprot Description

LASS1: May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked stearoyl- (C18) ceramide) in a fumonisin B1-independent manner. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p12

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: sphingosine N-acyltransferase activity

Biological Process: negative regulation of telomerase activity; sphingolipid metabolic process; sphingolipid biosynthetic process; ceramide biosynthetic process

Disease: Epilepsy, Progressive Myoclonic, 8