CELA1 sirna

CELA1 siRNA (Human)

Cat. Num. AAA8239049

• Gene Names: CELA1; ELA1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CELA1; CELA1 siRNA (Human); ELA1; Chymotrypsin-like elastase family member 1; Elastase-1; Pancreatic elastase 1; CELA1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CELA1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 258

• Applicable Applications for CELA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CELA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CELA1 sirna

siRNA to inhibit CELA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 58331209
• NCBI GeneID: 1990
• NCBI Accession #: NP_001962.3
• NCBI GenBank Nucleotide #: NM_001971.5
• UniProt Accession #: Q9UNI1; Q5MLF0; Q6DJT0; Q6ISM6
• Molecular Weight: 27,798 Da
• NCBI Official Full Name: chymotrypsin-like elastase family member 1
• NCBI Official Synonym Full Names: chymotrypsin-like elastase family, member 1
• NCBI Official Symbol: CELA1
• NCBI Official Synonym Symbols: ELA1
• NCBI Protein Information: chymotrypsin-like elastase family member 1
• UniProt Protein Name: Chymotrypsin-like elastase family member 1
• Protein Family: Chymotrypsin-like elastase family
• UniProt Gene Name: CELA1
• UniProt Synonym Gene Names: ELA1
• UniProt Entry Name: CELA1_HUMAN

NCBI Description

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B. [provided by RefSeq, May 2009]

Uniprot Description

CELA1: Acts upon elastin. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Secreted; Secreted, signal peptide; Protease; EC 3.4.21.36

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular region

Molecular Function: metal ion binding; serine-type endopeptidase activity

Biological Process: Wnt receptor signaling pathway; regulation of cell differentiation; multicellular organism growth; exocrine pancreas development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; inflammatory response; proteolysis; post-embryonic development; regulation of cell proliferation