Rabbit anti-Human CELA1 Polyclonal Antibody | anti-CELA1 antibody

CELA1, NT (CELA1, ELA1, Chymotrypsin-like elastase family member 1, Elastase-1, Pancreatic elastase 1) (Biotin)

Cat. Num. AAA6285009

• Gene Names: CELA1; ELA1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CELA1; Polyclonal Antibody; NT (CELA1; ELA1; Chymotrypsin-like elastase family member 1; Elastase-1; Pancreatic elastase 1) (Biotin); Pancreatic elastase 1; anti-CELA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CELA1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CELA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-79 amino acids from the N-terminal region of human CELA1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CELA1 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the CELA1 antibody detected the CELA1 protein (arrow).)

Related Product Information for anti-CELA1 antibody

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B.

Product Categories/Family for anti-CELA1 antibody

Antibodies; Elastase

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Talas, U., et al. J. Invest. Dermatol. 114(1):165-170(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 58331209
• NCBI GeneID: 1990
• NCBI Accession #: NP_001962.3
• NCBI GenBank Nucleotide #: NM_001971.5
• UniProt Accession #: Q9UNI1; Q5MLF0; Q6DJT0; Q6ISM6
• Molecular Weight: 27,798 Da
• NCBI Official Full Name: chymotrypsin-like elastase family member 1
• NCBI Official Synonym Full Names: chymotrypsin-like elastase family, member 1
• NCBI Official Symbol: CELA1
• NCBI Official Synonym Symbols: ELA1
• NCBI Protein Information: chymotrypsin-like elastase family member 1; elastase-1; pancreatic elastase 1; elastase 1, pancreatic
• UniProt Protein Name: Chymotrypsin-like elastase family member 1
• Protein Family: Chymotrypsin-like elastase family
• UniProt Gene Name: CELA1
• UniProt Synonym Gene Names: ELA1
• UniProt Entry Name: CELA1_HUMAN

NCBI Description

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B. [provided by RefSeq, May 2009]

Uniprot Description

CELA1: Acts upon elastin. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Protease; Secreted; Secreted, signal peptide; EC 3.4.21.36

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular region

Molecular Function: metal ion binding; serine-type endopeptidase activity

Biological Process: Wnt receptor signaling pathway; multicellular organism growth; regulation of cell differentiation; exocrine pancreas development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; proteolysis; inflammatory response; regulation of cell proliferation; post-embryonic development