CEBPA sirna

CEBPA siRNA (Human)

Cat. Num. AAA8221068

• Gene Names: CEBPA; CEBP; C/EBP-alpha
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CEBPA; CEBPA siRNA (Human); CCAAT/enhancer-binding protein alpha; C/EBP alpha; CEBPA sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CEBPA siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 393

• Applicable Applications for CEBPA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CEBPA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CEBPA sirna

siRNA to inhibit CEBPA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 551894998
• NCBI GeneID: 1050
• NCBI Accession #: NP_001272758.1
• NCBI GenBank Nucleotide #: NM_001285829.1
• UniProt Accession #: P49715; P78319; Q05CA4; A7LNP2
• Molecular Weight: 41,249 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein alpha isoform b
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), alpha
• NCBI Official Symbol: CEBPA
• NCBI Official Synonym Symbols: CEBP; C/EBP-alpha
• NCBI Protein Information: CCAAT/enhancer-binding protein alpha
• UniProt Protein Name: CCAAT/enhancer-binding protein alpha
• Protein Family: CCAAT/enhancer-binding protein
• UniProt Gene Name: CEBPA
• UniProt Synonym Gene Names: C/EBP alpha
• UniProt Entry Name: CEBPA_HUMAN

NCBI Description

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]

Uniprot Description

C/EBP-alpha: a bZIP transcription factor which can form homodimers or heterodimers with the related proteins CEBP-beta and CEBP-gamma. Binds to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: Rb-E2F complex; nuclear matrix; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; protein complex binding; transcription factor activity; transcription factor binding

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; embryonic placenta development; macrophage differentiation; viral reproduction; cell maturation; response to glucocorticoid stimulus; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; negative regulation of cell proliferation; response to vitamin B2; acute-phase response; inner ear development; cholesterol metabolic process; mitochondrion organization and biogenesis; generation of precursor metabolites and energy; organ regeneration; granulocyte differentiation; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; negative regulation of cyclin-dependent protein kinase activity; liver development; positive regulation of osteoblast differentiation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; white fat cell differentiation; brown fat cell differentiation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase III promoter; myeloid cell differentiation; positive regulation of transcription from RNA polymerase II promoter; lung development; urea cycle

Disease: Leukemia, Acute Myeloid