CDKAL1 sirna

CDKAL1 siRNA (Human)

Cat. Num. AAA8228366

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDKAL1; CDKAL1 siRNA (Human); Threonylcarbamoyladenosine tRNA methylthiotransferase; CDK5 regulatory subunit-associated protein 1-like 1; tRNA-t(6)A37 methylthiotransferase; CDKAL1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CDKAL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 579

• Applicable Applications for CDKAL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CDKAL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDKAL1 sirna

siRNA to inhibit CDKAL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 93277076
• NCBI GeneID: 54901
• NCBI Accession #: NP_060244.2
• NCBI GenBank Nucleotide #: NM_017774.3
• UniProt Accession #: Q5VV42; Q6P385; Q6ZR27; Q9NXB3; A8K6S0
• Molecular Weight: 11,043 Da
• NCBI Official Full Name: threonylcarbamoyladenosine tRNA methylthiotransferase
• NCBI Official Synonym Full Names: CDK5 regulatory subunit associated protein 1-like 1
• NCBI Official Symbol: CDKAL1
• NCBI Protein Information: threonylcarbamoyladenosine tRNA methylthiotransferase
• UniProt Protein Name: Threonylcarbamoyladenosine tRNA methylthiotransferase
• Protein Family: Threonylcarbamoyladenosine tRNA methylthiotransferase
• UniProt Gene Name: CDKAL1
• UniProt Entry Name: CDKAL_HUMAN

NCBI Description

The protein encoded by this gene is a member of the methylthiotransferase family. The function of this gene is not known. Genome-wide association studies have linked single nucleotide polymorphisms in an intron of this gene with susceptibilty to type 2 diabetes. [provided by RefSeq, May 2010]

Uniprot Description

CDKAL1: Catalyzes the methylthiolation of N6- threonylcarbamoyladenosine (t(6)A), leading to the formation of 2- methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine. Belongs to the methylthiotransferase family. CDKAL1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.8.4.5; Membrane protein, integral; Enzyme, misc.

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: endoplasmic reticulum membrane; membrane; rough endoplasmic reticulum; integral to membrane

Molecular Function: metal ion binding; 4 iron, 4 sulfur cluster binding

Disease: Diabetes Mellitus, Noninsulin-dependent