CDK5RAP2 sirna

CDK5RAP2 siRNA (Human)

Cat. Num. AAA8204263

• Gene Names: CDK5RAP2; C48; MCPH3; Cep215
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDK5RAP2; CDK5RAP2 siRNA (Human); CEP215; KIAA1633; CDK5 regulatory subunit-associated protein 2; CDK5 activator-binding protein C48; Centrosome-associated protein 215; CDK5RAP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CDK5RAP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1814

• Applicable Applications for CDK5RAP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CDK5RAP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDK5RAP2 sirna

siRNA to inhibit CDK5RAP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 58535453
• NCBI GeneID: 55755
• NCBI Accession #: NP_001011649.1
• NCBI GenBank Nucleotide #: NM_001011649.2
• UniProt Accession #: Q96SN8; Q5JV18; Q7Z3L4; Q7Z3U1; Q7Z7I6; Q9BSW0; Q9H6J6; Q9HCD9; Q9NV90; Q9UIW9
• Molecular Weight: 206,323 Da
• NCBI Official Full Name: CDK5 regulatory subunit-associated protein 2 isoform b
• NCBI Official Synonym Full Names: CDK5 regulatory subunit associated protein 2
• NCBI Official Symbol: CDK5RAP2
• NCBI Official Synonym Symbols: C48; MCPH3; Cep215
• NCBI Protein Information: CDK5 regulatory subunit-associated protein 2
• UniProt Protein Name: CDK5 regulatory subunit-associated protein 2
• Protein Family: CDK5 regulatory subunit-associated protein
• UniProt Gene Name: CDK5RAP2
• UniProt Synonym Gene Names: CEP215; KIAA1633
• UniProt Entry Name: CK5P2_HUMAN

NCBI Description

This gene encodes a regulator of CDK5 (cyclin-dependent kinase 5) activity. The protein encoded by this gene is localized to the centrosome and Golgi complex, interacts with CDK5R1 and pericentrin (PCNT), plays a role in centriole engagement and microtubule nucleation, and has been linked to primary microcephaly and Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

CDK5RAP2: Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation. Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with MAPRE1, it may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends. Defects in CDK5RAP2 are the cause of microcephaly primary type 3 (MCPH3). A disorder defined as a head circumference more than 3 standard deviations below the age- related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 9q33.2

Cellular Component: spindle pole; Golgi apparatus; centrosome; microtubule; pericentriolar material; cytoskeleton; perinuclear region of cytoplasm; cytoplasm; cell junction; cytosol

Molecular Function: tubulin binding; calmodulin binding; protein binding; microtubule binding; protein kinase binding

Biological Process: negative regulation of neuron differentiation; neurogenesis; establishment of mitotic spindle orientation; centrosome organization and biogenesis; regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; organelle organization and biogenesis; mitotic cell cycle; brain development; microtubule cytoskeleton organization and biogenesis; G2/M transition of mitotic cell cycle; negative regulation of centriole replication; microtubule bundle formation; chromosome segregation

Disease: Microcephaly 3, Primary, Autosomal Recessive