CDK5R2 sirna

CDK5R2 siRNA (Human)

Cat. Num. AAA8208511

• Gene Names: CDK5R2; P39; NCK5AI; p39nck5ai
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDK5R2; CDK5R2 siRNA (Human); NCK5AI; Cyclin-dependent kinase 5 activator 2; CDK5 activator 2; Cyclin-dependent kinase 5 regulatory subunit 2; p39; p39I; CDK5R2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CDK5R2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 367

• Applicable Applications for CDK5R2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CDK5R2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDK5R2 sirna

siRNA to inhibit CDK5R2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4502739
• NCBI GeneID: 8941
• NCBI Accession #: NP_003927.1
• NCBI GenBank Nucleotide #: NM_003936.4
• UniProt Accession #: Q13319; Q4ZFW6
• Molecular Weight: 38,705 Da
• NCBI Official Full Name: cyclin-dependent kinase 5 activator 2
• NCBI Official Synonym Full Names: cyclin-dependent kinase 5, regulatory subunit 2 (p39)
• NCBI Official Symbol: CDK5R2
• NCBI Official Synonym Symbols: P39; NCK5AI; p39nck5ai
• NCBI Protein Information: cyclin-dependent kinase 5 activator 2
• UniProt Protein Name: Cyclin-dependent kinase 5 activator 2
• Protein Family: Cyclin-dependent kinase 5 activator
• UniProt Gene Name: CDK5R2
• UniProt Synonym Gene Names: NCK5AI; CDK5 activator 2
• UniProt Entry Name: CD5R2_HUMAN

NCBI Description

The protein encoded by this gene is a neuron-specific activator of CDK5 kinase. It associates with CDK5 to form an active kinase. This protein and neuron-specific CDK5 activator CDK5R1/p39NCK5A both share limited similarity to cyclins, and thus may define a distinct family of cyclin-dependent kinase activating proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK5R2: Activator of CDK5/TPKII. Heterodimer of a catalytic subunit and a regulatory subunit. Brain and neuron specific. Belongs to the cyclin-dependent kinase 5 activator family.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: cyclin-dependent protein kinase 5 activator complex; cytoplasm; plasma membrane

Molecular Function: cyclin-dependent protein kinase 5 activator activity; lipid binding

Biological Process: positive regulation of calcium ion-dependent exocytosis; superior olivary nucleus maturation; hippocampus development; neuron migration; cerebellum development; regulation of cyclin-dependent protein kinase activity; layer formation in the cerebral cortex