CCT6B sirna

CCT6B siRNA (Human)

Cat. Num. AAA8226787

• Gene Names: CCT6B; Cctz2; CCTZ-2; TSA303; CCT-zeta-2; TCP-1-zeta-2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCT6B; CCT6B siRNA (Human); T-complex protein 1 subunit zeta-2; TCP-1-zeta-2; CCT-zeta-2; CCT-zeta-like; TCP-1-zeta-like; Testis-specific Tcp20; Testis-specific protein TSA303; CCT6B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CCT6B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 493

• Applicable Applications for CCT6B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CCT6B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCT6B sirna

siRNA to inhibit CCT6B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 302058290
• NCBI GeneID: 10693
• NCBI Accession #: NP_001180458.1
• NCBI GenBank Nucleotide #: NM_001193529.2
• UniProt Accession #: Q92526; Q8TC34; B4DX20; B4DYB0
• Molecular Weight: 53,586 Da
• NCBI Official Full Name: T-complex protein 1 subunit zeta-2 isoform 2
• NCBI Official Synonym Full Names: chaperonin containing TCP1, subunit 6B (zeta 2)
• NCBI Official Symbol: CCT6B
• NCBI Official Synonym Symbols: Cctz2; CCTZ-2; TSA303; CCT-zeta-2; TCP-1-zeta-2
• NCBI Protein Information: T-complex protein 1 subunit zeta-2
• UniProt Protein Name: T-complex protein 1 subunit zeta-2
• Protein Family: T-complex protein
• UniProt Gene Name: CCT6B
• UniProt Synonym Gene Names: TCP-1-zeta-2
• UniProt Entry Name: TCPW_HUMAN

NCBI Description

This gene encodes a molecular chaperone that is a member of the chaperonin-containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]

Uniprot Description

CCT-zeta-2: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. Belongs to the TCP-1 chaperonin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: chaperonin-containing T-complex

Molecular Function: unfolded protein binding; ATP binding; protein transporter activity

Biological Process: protein transport; protein folding; spermatogenesis; chaperone-mediated protein complex assembly