CCDC50 sirna

CCDC50 siRNA (Human)

Cat. Num. AAA8213865

• Gene Names: CCDC50; YMER; C3orf6; DFNA44
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCDC50; CCDC50 siRNA (Human); C3orf6; Coiled-coil domain-containing protein 50; Protein Ymer; CCDC50 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CCDC50 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 306

• Applicable Applications for CCDC50 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CCDC50 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCDC50 sirna

siRNA to inhibit CCDC50 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 28372509
• NCBI GeneID: 152137
• NCBI Accession #: NP_777568.1
• NCBI GenBank Nucleotide #: NM_174908.3
• UniProt Accession #: Q8IVM0; Q86VH7
• Molecular Weight: 56,340 Da
• NCBI Official Full Name: coiled-coil domain-containing protein 50 short isoform
• NCBI Official Synonym Full Names: coiled-coil domain containing 50
• NCBI Official Symbol: CCDC50
• NCBI Official Synonym Symbols: YMER; C3orf6; DFNA44
• NCBI Protein Information: coiled-coil domain-containing protein 50
• UniProt Protein Name: Coiled-coil domain-containing protein 50
• Protein Family: Coiled-coil domain-containing protein
• UniProt Gene Name: CCDC50
• UniProt Synonym Gene Names: C3orf6
• UniProt Entry Name: CCD50_HUMAN

NCBI Description

This gene encodes a soluble, cytoplasmic, tyrosine-phosphorylated protein with multiple ubiquitin-interacting domains. Mutations in this gene cause nonsyndromic, postlingual, progressive sensorineural DFNA44 hearing loss. In mouse, the protein is expressed in the inner ear during development and postnatal maturation and associates with microtubule-based structures. This protein may also function as a negative regulator of NF-kB signaling and as an effector of epidermal growth factor (EGF)-mediated cell signaling. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

CCDC50 iso2: phosphorylated in response to EGFR signaling in human mammary epithelial cells. Two alternatively-spliced isoforms have been described.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: cytoplasm

Molecular Function: protein binding; ubiquitin protein ligase binding

Disease: Deafness, Autosomal Dominant 44