CCDC101 sirna

CCDC101 siRNA (Human)

Cat. Num. AAA8238483

• Gene Names: SGF29; STAF36; CCDC101
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCDC101; CCDC101 siRNA (Human); SGF29; SAGA-associated factor 29 homolog; Coiled-coil domain-containing protein 101; CCDC101 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CCDC101 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 293

• Applicable Applications for CCDC101 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CCDC101 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCDC101 sirna

siRNA to inhibit CCDC101 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19923935
• NCBI GeneID: 112869
• NCBI Accession #: NP_612423.1
• NCBI GenBank Nucleotide #: NM_138414.2
• UniProt Accession #: Q96ES7; Q96MF5
• Molecular Weight: 33,238 Da
• NCBI Official Full Name: SAGA-associated factor 29 homolog
• NCBI Official Synonym Full Names: SAGA complex associated factor 29
• NCBI Official Symbol: SGF29
• NCBI Official Synonym Symbols: STAF36; CCDC101
• NCBI Protein Information: SAGA-associated factor 29 homolog
• UniProt Protein Name: SAGA-associated factor 29 homolog
• UniProt Gene Name: CCDC101
• UniProt Synonym Gene Names: SGF29
• UniProt Entry Name: SGF29_HUMAN

NCBI Description

CCDC101 is a subunit of 2 histone acetyltransferase complexes: the ADA2A (TADA2A; MIM 602276)-containing (ATAC) complex and the SPT3 (SUPT3H; MIM 602947)-TAF9 (MIM 600822)-GCN5 (KAT2A; MIM 602301)/PCAF (KAT2B; MIM 602303) acetylase (STAGA) complex. Both of these complexes contain either GCN5 or PCAF, which are paralogous acetyltransferases (Wang et al., 2008 [PubMed 18838386]).[supplied by OMIM, Apr 2010]

Uniprot Description

CCDC101: Involved in transcriptional regulation, through association with histone acetyltransferase (HAT) SAGA-type complexes like the TFTC-HAT, ATAC or STAGA complexes. Specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3). In the SAGA- type complexes, required to recruit complexes to H3K4me. May be involved in MYC-mediated oncogenic transformation. Belongs to the SGF29 family.

Chromosomal Location of Human Ortholog: 16p11.2

Molecular Function: protein binding; methylated histone residue binding

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent