CASP8AP2 sirna

CASP8AP2 siRNA (Human)

Cat. Num. AAA8212022

• Gene Names: CASP8AP2; CED-4; FLASH; RIP25
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CASP8AP2; CASP8AP2 siRNA (Human); FLASH; KIAA1315; RIP25; CASP8-associated protein 2; FLICE-associated huge protein; CASP8AP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CASP8AP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1982

• Applicable Applications for CASP8AP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CASP8AP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CASP8AP2 sirna

siRNA to inhibit CASP8AP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 212549782
• NCBI GeneID: 9994
• NCBI Accession #: NP_001131139.1
• NCBI GenBank Nucleotide #: NM_001137667.1
• UniProt Accession #: Q9UKL3; Q6PH76; Q7LCQ7; Q86YD9; Q9NUQ4; Q9NZV9; Q9P2N1; Q9Y563; A2RUB7; E1P553
• Molecular Weight: 222,658 Da
• NCBI Official Full Name: CASP8-associated protein 2
• NCBI Official Synonym Full Names: caspase 8 associated protein 2
• NCBI Official Symbol: CASP8AP2
• NCBI Official Synonym Symbols: CED-4; FLASH; RIP25
• NCBI Protein Information: CASP8-associated protein 2
• UniProt Protein Name: CASP8-associated protein 2
• Protein Family: CASP8-associated protein
• UniProt Gene Name: CASP8AP2
• UniProt Entry Name: C8AP2_HUMAN

NCBI Description

This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Nov 2008]

Uniprot Description

CASP8AP2: Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF- kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase.

Protein type: Apoptosis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6q15

Cellular Component: PML body; mitochondrion; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; death receptor binding; SUMO polymer binding; apoptotic protease activator activity; caspase activator activity; transcription corepressor activity

Biological Process: caspase activation; transcription, DNA-dependent; induction of apoptosis via death domain receptors; regulation of transcription, DNA-dependent; signal transduction; cell cycle