Rabbit anti-Human, Mouse TBX15 Polyclonal Antibody | anti-TBX15 antibody

TBX15, NT (TBX15, TBX14, T-box transcription factor TBX15, T-box transcription factor TBX14) (Biotin)

Cat. Num. AAA6354163

• Gene Names: TBX15; TBX14
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TBX15; Polyclonal Antibody; NT (TBX15; TBX14; T-box transcription factor TBX15; T-box transcription factor TBX14) (Biotin); T-box transcription factor TBX14; anti-TBX15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TBX15 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TBX15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-73 amino acids from the N-terminal region of human TBX15.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Fluorescent image of HUVEC cell stained with TBX15 Antibody (N-term).HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX15 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).TBX15 immunoreactivity is localized to Nucleus significantly. )

Western Blot (WB)

(TBX15 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the TBX15 antibody detected the TBX15 protein (arrow).)

Related Product Information for anti-TBX15 antibody

Probable transcriptional regulator involved in developmental processes.

Product Categories/Family for anti-TBX15 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 55770860
• NCBI GeneID: 6913
• NCBI Accession #: NP_689593.2
• NCBI GenBank Nucleotide #: NM_152380.2
• UniProt Accession #: Q96SF7; Q08E76; Q5JT54; Q5T9S7
• Molecular Weight: 54,671 Da
• NCBI Official Full Name: T-box transcription factor TBX15
• NCBI Official Synonym Full Names: T-box 15
• NCBI Official Symbol: TBX15
• NCBI Official Synonym Symbols: TBX14
• NCBI Protein Information: T-box transcription factor TBX15; T-box 14; T-box protein 14; T-box protein 15; T-box transcription factor TBX14
• UniProt Protein Name: T-box transcription factor TBX15
• Protein Family: T-box transcription factor
• UniProt Gene Name: TBX15
• UniProt Synonym Gene Names: TBX14; T-box protein 15; T-box protein 14
• UniProt Entry Name: TBX15_HUMAN

NCBI Description

This gene belongs to the T-box family of genes, which encode a phylogenetically conserved family of transcription factors that regulate a variety of developmental processes. All these genes contain a common T-box DNA-binding domain. Mutations in this gene are associated with Cousin syndrome.[provided by RefSeq, Oct 2009]

Uniprot Description

TBX15: Probable transcriptional regulator involved in the development of the skeleton of the limb, vertebral column and head. Acts by controlling the number of mesenchymal precursor cells and chondrocytes. Defects in TBX15 are the cause of Cousin syndrome (COUSS); also known as craniofacial dysmorphism, hypoplasia of scapula and pelvis, and short stature. Cousin syndrome is defined as pelviscapular dysplasia with epiphyseal abnormalities, congenital dwarfism and facial dysmorphy (frontal bossing, hypertelorism, narrow palpebral fissures, deep set globes, strabismus, low-set posteriory rotated and unusually formed external ears, dysplasia of conchae, small chin, short neck with redundant skin folds, and a low hairline). Intelligence may vary from normal to moderately impaired. Radiographic features comprise aplasia of the body of the scapula, hypoplasia of the iliac bone, humeroradial synosthosis, dislocation of the femoral heads, and moderate brachydactyly. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1p11.1

Molecular Function: protein homodimerization activity; protein heterodimerization activity; transcription factor activity

Biological Process: transcription, DNA-dependent; embryonic cranial skeleton morphogenesis; negative regulation of transcription from RNA polymerase II promoter

Disease: Cousin Syndrome