BLM sirna

BLM siRNA (Human)

Cat. Num. AAA8235486

• Gene Names: BLM; BS; RECQ2; RECQL2; RECQL3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BLM; BLM siRNA (Human); RECQ2; RECQL3; Bloom syndrome protein; DNA helicase. RecQ-like type 2; RecQ2; RecQ protein-like 3; BLM sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: BLM siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1417

• Applicable Applications for BLM sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BLM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BLM sirna

siRNA to inhibit BLM expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4557365
• NCBI GeneID: 641
• NCBI Accession #: NP_000048.1
• NCBI GenBank Nucleotide #: NM_000057.3
• UniProt Accession #: P54132; Q52M96
• Molecular Weight: 159,000 Da
• NCBI Official Full Name: Bloom syndrome protein isoform 1
• NCBI Official Synonym Full Names: Bloom syndrome, RecQ helicase-like
• NCBI Official Symbol: BLM
• NCBI Official Synonym Symbols: BS; RECQ2; RECQL2; RECQL3
• NCBI Protein Information: Bloom syndrome protein
• UniProt Protein Name: Bloom syndrome protein
• Protein Family: Bloom syndrome protein
• UniProt Gene Name: BLM
• UniProt Synonym Gene Names: RECQ2; RECQL3; RecQ2
• UniProt Entry Name: BLM_HUMAN

NCBI Description

The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008]

Uniprot Description

BLM: a magnesium-dependent ATP-dependent DNA-helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. A member of the RecQ helicase family that is required for genome stability. Participates in DNA replication, recombination and repair. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex which is a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2.

Protein type: DNA repair, damage; DNA replication; Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: chromosome, telomeric region; cytoplasm; lateral element; nuclear chromosome; nuclear matrix; nucleolus; nucleoplasm; nucleus; PML body

Molecular Function: ATP binding; ATP-dependent 3'-5' DNA helicase activity; ATP-dependent DNA helicase activity; ATP-dependent helicase activity; ATPase activity; bubble DNA binding; four-way junction helicase activity; G-quadruplex DNA binding; helicase activity; p53 binding; protein binding; single-stranded DNA binding

Biological Process: cellular protein metabolic process; DNA double-strand break processing; DNA duplex unwinding; DNA recombination; DNA repair; DNA strand renaturation; double-strand break repair; double-strand break repair via homologous recombination; double-strand break repair via synthesis-dependent strand annealing; mitotic cell cycle G2/M transition DNA damage checkpoint; negative regulation of cell division; negative regulation of DNA recombination; positive regulation of transcription, DNA-dependent; post-translational protein modification; protein oligomerization; protein sumoylation; regulation of cyclin-dependent protein kinase activity; replication fork processing; replication fork protection; response to DNA damage stimulus; response to X-ray

Disease: Bloom Syndrome