BHLHE40 sirna

BHLHE40 siRNA (Human)

Cat. Num. AAA8212655

• Gene Names: BHLHE40; DEC1; HLHB2; BHLHB2; STRA13; Stra14; SHARP-2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BHLHE40; BHLHE40 siRNA (Human); BHLHB2; DEC1; SHARP2; STRA13; Class E basic helix-loop-helix protein 40; bHLHe40; Class B basic helix-loop-helix protein 2; bHLHb2; Differentially expressed in chondrocytes protein 1; Enhancer-of-split and hairy-related protein 2; SHARP-2; Stimulated by retinoic acid gene 13 protein; BHLHE40 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: BHLHE40 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 412

• Applicable Applications for BHLHE40 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BHLHE40 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BHLHE40 sirna

siRNA to inhibit BHLHE40 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4503299
• NCBI GeneID: 8553
• NCBI Accession #: NP_003661.1
• NCBI GenBank Nucleotide #: NM_003670.2
• UniProt Accession #: O14503; Q96TD3
• Molecular Weight: 45,510 Da
• NCBI Official Full Name: class E basic helix-loop-helix protein 40
• NCBI Official Synonym Full Names: basic helix-loop-helix family, member e40
• NCBI Official Symbol: BHLHE40
• NCBI Official Synonym Symbols: DEC1; HLHB2; BHLHB2; STRA13; Stra14; SHARP-2
• NCBI Protein Information: class E basic helix-loop-helix protein 40
• UniProt Protein Name: Class E basic helix-loop-helix protein 40
• Protein Family: Class E basic helix-loop-helix protein
• UniProt Gene Name: BHLHE40
• UniProt Synonym Gene Names: BHLHB2; DEC1; SHARP2; STRA13; bHLHe40; bHLHb2; DEC1; SHARP-2
• UniProt Entry Name: BHE40_HUMAN

NCBI Description

This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. [provided by RefSeq, Feb 2014]

Uniprot Description

BHLHB2: May function as a transcriptional factor to modulate chondrogenesis in response to the cAMP pathway.

Protein type: Nucleolus; Transcription regulation

Chromosomal Location of Human Ortholog: 3p26

Cellular Component: nucleoplasm; Golgi apparatus; nucleolus; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein domain specific binding; MRF binding; protein binding; protein homodimerization activity; protein heterodimerization activity; bHLH transcription factor binding; transcription corepressor activity; transcription factor activity

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; circadian regulation of gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; entrainment of circadian clock by photoperiod