BFSP2 sirna

BFSP2 siRNA (Human)

Cat. Num. AAA8230771

• Gene Names: BFSP2; CP47; CP49; LIFL-L; CTRCT12; PHAKOSIN
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BFSP2; BFSP2 siRNA (Human); Phakinin; 49 kDa cytoskeletal protein; Beaded filament structural protein 2; Lens fiber cell beaded filament protein CP 47; CP47; Lens fiber cell beaded filament protein CP 49; CP49; Lens intermediate filament-like light; LIFL-L; BFSP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: BFSP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 415

• Applicable Applications for BFSP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BFSP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BFSP2 sirna

siRNA to inhibit BFSP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4502995
• NCBI GeneID: 8419
• NCBI Accession #: NP_003562.1
• NCBI GenBank Nucleotide #: NM_003571.3
• UniProt Accession #: Q13515; Q14D32; Q9HBW5
• Molecular Weight: 45,880 Da
• NCBI Official Full Name: phakinin
• NCBI Official Synonym Full Names: beaded filament structural protein 2, phakinin
• NCBI Official Symbol: BFSP2
• NCBI Official Synonym Symbols: CP47; CP49; LIFL-L; CTRCT12; PHAKOSIN
• NCBI Protein Information: phakinin
• UniProt Protein Name: Phakinin
• Protein Family: Phakinin
• UniProt Gene Name: BFSP2
• UniProt Synonym Gene Names: CP47; CP49; LIFL-L
• UniProt Entry Name: BFSP2_HUMAN

NCBI Description

More than 99% of the vertebrate ocular lens is comprised of terminally differentiated lens fiber cells. Two lens-specific intermediate filament-like proteins, the protein product of this gene (phakinin), and filensin, are expressed only after fiber cell differentiation has begun. Both proteins are found in a structurally unique cytoskeletal element that is referred to as the beaded filament (BF). Mutations in this gene have been associated with juvenile-onset, progressive cataracts and Dowling-Meara epidermolysis bullosa simplex. [provided by RefSeq, Jun 2009]

Uniprot Description

BFSP2: Involved in stabilization of lens fiber cell cytoskeleton. Defects in BFSP2 are the cause of cataract autosomal dominant multiple types 1 (ADC-MT1). Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. The opacities can be nuclear, sutural, stellate cortical, lamellar, cortical, nuclear embryonic, Y-sutural, punctate cortical, congenital or with juvenile- and adult-onset. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: cytoplasm; plasma membrane; intermediate filament

Molecular Function: structural constituent of cytoskeleton; structural constituent of eye lens

Biological Process: visual perception; response to stimulus; cell maturation; intermediate filament cytoskeleton organization and biogenesis

Disease: Cataract 12, Multiple Types