BAAT sirna

BAAT siRNA (Human)

Cat. Num. AAA8205182

• Gene Names: BAAT; BAT; BACAT
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BAAT; BAAT siRNA (Human); Bile acid-CoA:amino acid N-acyltransferase; BACAT; BAT; Glycine N-choloyltransferase; Long-chain fatty-acyl-CoA hydrolase; BAAT sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: BAAT siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 418

• Applicable Applications for BAAT sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BAAT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BAAT sirna

siRNA to inhibit BAAT expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 189083866
• NCBI GeneID: 570
• NCBI Accession #: NP_001121082.1
• NCBI GenBank Nucleotide #: NM_001127610.1
• UniProt Accession #: Q14032; Q3B7W9; Q96L31
• Molecular Weight: 46,299 Da
• NCBI Official Full Name: bile acid-CoA:amino acid N-acyltransferase
• NCBI Official Synonym Full Names: bile acid CoA:amino acid N-acyltransferase
• NCBI Official Symbol: BAAT
• NCBI Official Synonym Symbols: BAT; BACAT
• NCBI Protein Information: bile acid-CoA:amino acid N-acyltransferase
• UniProt Protein Name: Bile acid-CoA:amino acid N-acyltransferase
• Protein Family: Bile acid-CoA:amino acid N-acyltransferase
• UniProt Gene Name: BAAT
• UniProt Synonym Gene Names: BACAT; BAT
• UniProt Entry Name: BAAT_HUMAN

NCBI Description

The protein encoded by this gene is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BAAT: Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs. Defects in BAAT are involved in familial hypercholanemia (FHCA). FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption. Belongs to the C/M/P thioester hydrolase family.

Protein type: EC 2.3.1.65; Transferase; Lipid Metabolism - primary bile acid biosynthesis; Other Amino Acids Metabolism - taurine and hypotaurine; Hydrolase; Lipid Metabolism - unsaturated fatty acid biosynthesis; EC 3.1.2.2

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: peroxisomal matrix; peroxisome; cytosol

Molecular Function: N-acyltransferase activity; glycine N-choloyltransferase activity; palmitoyl-CoA hydrolase activity; receptor binding

Biological Process: bile acid and bile salt transport; bile acid biosynthetic process; glycine metabolic process; organ regeneration; acyl-CoA metabolic process; bile acid metabolic process; fatty acid metabolic process; liver development; taurine metabolic process

Disease: Hypercholanemia, Familial