ATP5G3 sirna

ATP5G3 siRNA (Human)

Cat. Num. AAA8225703

• Gene Names: ATP5G3; P3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATP5G3; ATP5G3 siRNA (Human); ATP synthase F(0) complex subunit C3 mitochondrial; ATP synthase lipid-binding protein; ATP synthase proteolipid P3; ATP synthase proton-transporting mitochondrial F(0) complex subunit C3; ATPase protein 9; ATPase subunit c; ATP5G3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ATP5G3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 142

• Applicable Applications for ATP5G3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ATP5G3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATP5G3 sirna

siRNA to inhibit ATP5G3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 50659074
• NCBI GeneID: 518
• NCBI Accession #: NP_001002258.1
• NCBI GenBank Nucleotide #: NM_001002258.4
• UniProt Accession #: P48201; Q4ZFX7; B2R4Z0; D3DPF0
• Molecular Weight: 14,693 Da
• NCBI Official Full Name: ATP synthase F(0) complex subunit C3, mitochondrial isoform A
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C3 (subunit 9)
• NCBI Official Symbol: ATP5G3
• NCBI Official Synonym Symbols: P3
• NCBI Protein Information: ATP synthase F(0) complex subunit C3, mitochondrial
• UniProt Protein Name: ATP synthase F(0) complex subunit C3, mitochondrial
• UniProt Gene Name: ATP5G3
• UniProt Entry Name: AT5G3_HUMAN

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding different proteins have been identified. [provided by RefSeq, Jun 2010]

Uniprot Description

ATP5G3: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element. Belongs to the ATPase C chain family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: mitochondrial outer membrane; proton-transporting ATP synthase complex, coupling factor F(o); integral to membrane; mitochondrial proton-transporting ATP synthase complex

Molecular Function: transporter activity; hydrogen ion transmembrane transporter activity; lipid binding

Biological Process: cellular metabolic process; ATP hydrolysis coupled proton transport; mitochondrial ATP synthesis coupled proton transport