Rabbit anti-Human ATP5G3 Polyclonal Antibody | anti-ATP5G3 antibody

ATP5G3 Antibody

Cat. Num. AAA9403203

• Gene Names: ATP5G3; P3
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: ATP5G3; Polyclonal Antibody; ATP5G3 Antibody; ATP synthase lipid-binding protein; mitochondrial; ATP synthase proteolipid P3; ATPase protein 9; ATPase subunit c; anti-ATP5G3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total ATP5G3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 142

• Applicable Applications for anti-ATP5G3 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:500
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human ATP5G3.
• Target Name: ATP5G3
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using ATP5G3 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells, using ATP5G3 antibody.)

Related Product Information for anti-ATP5G3 antibody

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element. HAMAP-Rule MF_01396Yan W.L., Genomics 24:375-377(1994).L., Du H., Nature 434:724-731(2005).The MGC Project Team; Genome Res. 14:2121-2127(2004).

Product Categories/Family for anti-ATP5G3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 50659074
• NCBI GeneID: 518
• NCBI Accession #: NP_001002258.1
• NCBI GenBank Nucleotide #: NM_001002258.4
• UniProt Accession #: P48201; Q4ZFX7; B2R4Z0; D3DPF0
• Molecular Weight: 14,693 Da
• NCBI Official Full Name: ATP synthase F(0) complex subunit C3, mitochondrial isoform A
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C3 (subunit 9)
• NCBI Official Symbol: ATP5G3
• NCBI Official Synonym Symbols: P3
• NCBI Protein Information: ATP synthase F(0) complex subunit C3, mitochondrial
• UniProt Protein Name: ATP synthase F(0) complex subunit C3, mitochondrial
• UniProt Gene Name: ATP5G3
• UniProt Entry Name: AT5G3_HUMAN

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding different proteins have been identified. [provided by RefSeq, Jun 2010]

Uniprot Description

ATP5G3: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element. Belongs to the ATPase C chain family.

Protein type: Energy Metabolism - oxidative phosphorylation; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: mitochondrial outer membrane; proton-transporting ATP synthase complex, coupling factor F(o); integral to membrane; mitochondrial proton-transporting ATP synthase complex

Molecular Function: transporter activity; hydrogen ion transmembrane transporter activity; lipid binding

Biological Process: cellular metabolic process; ATP hydrolysis coupled proton transport; mitochondrial ATP synthesis coupled proton transport