ATMIN sirna

ATMIN siRNA (Human)

Cat. Num. AAA8237014

• Gene Names: ATMIN; ASCIZ; ZNF822
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATMIN; ATMIN siRNA (Human); KIAA0431; ZNF822; ATM interactor; ATM/ATR-substrate CHK2-interacting zinc finger protein; ASCIZ; Zinc finger protein 822; ATMIN sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ATMIN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 667

• Applicable Applications for ATMIN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ATMIN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATMIN sirna

siRNA to inhibit ATMIN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 663429532
• NCBI GeneID: 23300
• NCBI Accession #: NP_001287657.1
• NCBI GenBank Nucleotide #: NM_001300728.1
• UniProt Accession #: O43313; Q68DC9; A8K4H8
• Molecular Weight: 72,334 Da
• NCBI Official Full Name: ATM interactor isoform 2
• NCBI Official Synonym Full Names: ATM interactor
• NCBI Official Symbol: ATMIN
• NCBI Official Synonym Symbols: ASCIZ; ZNF822
• NCBI Protein Information: ATM interactor
• UniProt Protein Name: ATM interactor
• Protein Family: ATM interactor
• UniProt Gene Name: ATMIN
• UniProt Synonym Gene Names: KIAA0431; ZNF822; ASCIZ
• UniProt Entry Name: ATMIN_HUMAN

Uniprot Description

ATMIN: Transcription factor. Plays a crucial role in cell survival and RAD51 foci formation in response to methylating DNA damage. Involved in regulating the activity of ATM in the absence of DNA damage. May play a role in stabilizing ATM. Binds to the DYNLL1 promoter and activates its transcription. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; DNA repair, damage

Chromosomal Location of Human Ortholog: 16q23.2

Cellular Component: nucleus

Molecular Function: dynein binding; protein binding; metal ion binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; response to DNA damage stimulus