ASCL1 sirna

ASCL1 siRNA (Human)

Cat. Num. AAA8222469

• Gene Names: ASCL1; ASH1; HASH1; MASH1; bHLHa46
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ASCL1; ASCL1 siRNA (Human); ASH1; BHLHA46; HASH1; Achaete-scute homolog 1; ASH-1; hASH1; Class A basic helix-loop-helix protein 46; bHLHa46; ASCL1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ASCL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 236

• Applicable Applications for ASCL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ASCL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ASCL1 sirna

siRNA to inhibit ASCL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 55743094
• NCBI GeneID: 429
• NCBI Accession #: NP_004307.2
• NCBI GenBank Nucleotide #: NM_004316.3
• UniProt Accession #: P50553; Q9BQ30; A8K3C4
• Molecular Weight: 25,454 Da
• NCBI Official Full Name: achaete-scute homolog 1
• NCBI Official Synonym Full Names: achaete-scute family bHLH transcription factor 1
• NCBI Official Symbol: ASCL1
• NCBI Official Synonym Symbols: ASH1; HASH1; MASH1; bHLHa46
• NCBI Protein Information: achaete-scute homolog 1
• UniProt Protein Name: Achaete-scute homolog 1
• Protein Family: Achaete-scute
• UniProt Gene Name: ASCL1
• UniProt Synonym Gene Names: ASH1; BHLHA46; HASH1; ASH-1; hASH1; bHLHa46
• UniProt Entry Name: ASCL1_HUMAN

NCBI Description

This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq, Jul 2008]

Uniprot Description

ASCL1: Transcriptional regulator. May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Involved in the initiation of neuronal differentiation. Mediates transcription activation by binding to the E box (5'-CANNTG-3').

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q23.2

Cellular Component: cell soma; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; double-stranded DNA binding; bHLH transcription factor binding; chromatin binding; transcription factor activity

Biological Process: vestibular nucleus development; response to lithium ion; musculoskeletal movement, spinal reflex action; cell maturation; olfactory pit development; regulation of mitotic cell cycle; neuron migration; neuroblast fate determination; negative regulation of transcription from RNA polymerase II promoter; sympathetic nervous system development; positive regulation of neuron apoptosis; response to folic acid; neuron fate specification; oligodendrocyte development; positive regulation of Notch signaling pathway; central nervous system neuron development; Notch signaling pathway; response to retinoic acid; transcription, DNA-dependent; spinal cord oligodendrocyte cell fate specification; positive regulation of cell cycle; regulation of timing of subpallium neuron differentiation; cerebral cortex GABAergic interneuron differentiation; negative regulation of neuron differentiation; neurogenesis; subpallium neuron fate commitment; commitment of a neuronal cell to a specific type of neuron in the forebrain; regulation of gene expression; neuroblast proliferation; regulation of epithelial cell differentiation; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; positive regulation of neuron differentiation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

Disease: Central Hypoventilation Syndrome, Congenital