ASB4 sirna

ASB4 siRNA (Human)

Cat. Num. AAA8209639

• Gene Names: ASB4; ASB-4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ASB4; ASB4 siRNA (Human); Ankyrin repeat and SOCS box protein 4; ASB-4; ASB4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ASB4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 426

• Applicable Applications for ASB4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ASB4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ASB4 sirna

siRNA to inhibit ASB4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7706379
• NCBI GeneID: 51666
• NCBI Accession #: NP_057200.1
• NCBI GenBank Nucleotide #: NM_016116.2
• UniProt Accession #: Q9Y574; O14586; Q14D68; Q8TBT2; A4D1H6
• Molecular Weight: 38,931 Da
• NCBI Official Full Name: ankyrin repeat and SOCS box protein 4 isoform a
• NCBI Official Synonym Full Names: ankyrin repeat and SOCS box containing 4
• NCBI Official Symbol: ASB4
• NCBI Official Synonym Symbols: ASB-4
• NCBI Protein Information: ankyrin repeat and SOCS box protein 4
• UniProt Protein Name: Ankyrin repeat and SOCS box protein 4
• Protein Family: Ankyrin repeat and SOCS box protein
• UniProt Gene Name: ASB4
• UniProt Synonym Gene Names: ASB-4
• UniProt Entry Name: ASB4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. They contain ankyrin repeat sequence and SOCS box domain. The SOCS box serves to couple suppressor of cytokine signalling (SOCS) proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation. Multiple alternatively spliced transcript variants have been described for this gene but some of the full length sequences are not known. [provided by RefSeq, Jul 2008]

Uniprot Description

ASB4: Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes differentiation and maturation of the vascular lineage by an oxygen-dependent mechanism. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 7q21-q22

Cellular Component: Cul2-RING ubiquitin ligase complex; Cul5-RING ubiquitin ligase complex

Molecular Function: ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: protein autoubiquitination