Western Blot (WB)
((0.1ug/ml) staining of lysate of cell line A549 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat IRF5 Polyclonal Antibody | anti-IRF5 antibody
Goat anti-IRF5 Antibody
Immunohistochemistry: This antibody showed nuclear staining in Human Cervix, as confirmed by anonymous customer (data not shown).
Western Blot: Approx 57kDa band observed in lysates of cell line A549. In transfected HEK293 transiently expressing IRF5 a strong band of approx. 60kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 57.8kDa according to NP_001092099.1. Recommended concentration, 0.1-0.3ug/ml.
Chromatin immunoprecipitation: This antibody has been successfully used in human monocyte-derived dendritic cells.
Western Blot (WB)
((0.1ug/ml) staining of lysate of cell line A549 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of lysate of cell line A549 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing human IRF5 and probed at 1ug/ml (mock transfection in lane B).)
Western Blot (WB)
(HEK293 overexpressing human IRF5 and probed at 1ug/ml (mock transfection in lane B).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Mar 2010]
Uniprot Description
IRF5: Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling. Homodimer, when phosphorylated. Belongs to the IRF family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 7q32
Cellular Component: cytoplasm; cytosol; nucleus
Molecular Function: protein binding; transcription factor activity
Biological Process: defense response to virus; positive regulation of apoptosis; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; positive regulation of interleukin-12 production; positive regulation of transcription from RNA polymerase II promoter; response to muramyl dipeptide; response to peptidoglycan; transcription, DNA-dependent
Disease: Inflammatory Bowel Disease 14; Systemic Lupus Erythematosus, Susceptibility To, 10
Research Articles on IRF5
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Product Notes
The IRF5 irf5 (Catalog #AAA421517) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-IRF5 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's IRF5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: This antibody showed nuclear staining in Human Cervix, as confirmed by anonymous customer (data not shown). Western Blot: Approx 57kDa band observed in lysates of cell line A549. In transfected HEK293 transiently expressing IRF5 a strong band of approx. 60kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 57.8kDa according to NP_001092099.1. Recommended concentration, 0.1-0.3ug/ml. Chromatin immunoprecipitation: This antibody has been successfully used in human monocyte-derived dendritic cells. Researchers should empirically determine the suitability of the IRF5 irf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QGPWPMHPAG MQ. It is sometimes possible for the material contained within the vial of "IRF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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