AP1S3 sirna

AP1S3 siRNA (Human)

Cat. Num. AAA8217544

• Gene Names: AP1S3; PSORS15
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AP1S3; AP1S3 siRNA (Human); AP-1 complex subunit sigma-3; Adapter-related protein complex 1 subunit sigma-1C; Adaptor protein complex AP-1 subunit sigma-1C; Clathrin assembly protein complex 1 sigma-1C small chain; Golgi adaptor HA1/AP1 adaptin sigma-1C subunit; Sigma 1C subunit of AP-1 clathrin; Sigma-adaptin 1C; Sigma1C-adaptin; AP1S3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AP1S3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 104

• Applicable Applications for AP1S3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AP1S3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AP1S3 sirna

siRNA to inhibit AP1S3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 88703051
• NCBI GeneID: 130340
• NCBI Accession #: NP_001034658.1
• NCBI GenBank Nucleotide #: NM_001039569.1
• UniProt Accession #: Q96PC3; Q8WTY1; Q96DD1; B4DQZ1
• Molecular Weight: 12,655 Da
• NCBI Official Full Name: AP-1 complex subunit sigma-3
• NCBI Official Synonym Full Names: adaptor-related protein complex 1, sigma 3 subunit
• NCBI Official Symbol: AP1S3
• NCBI Official Synonym Symbols: PSORS15
• NCBI Protein Information: AP-1 complex subunit sigma-3
• UniProt Protein Name: AP-1 complex subunit sigma-3
• Protein Family: AP-1 complex
• UniProt Gene Name: AP1S3
• UniProt Entry Name: AP1S3_HUMAN

NCBI Description

This gene encodes a member of the adaptor-related protein complex 1, sigma subunit genes. The encoded protein is a component of adaptor protein complex 1 (AP-1), one of the AP complexes involved in claathrin-mediated vesicular transport from the Golgi or endosomes. Disruption of the pathway for display of HIV-1 antigens, which prevents recognition of the virus by cytotoxic T cells, has been shown to involve the AP-1 complex (PMID: 15569716). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

AP1S3: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. Belongs to the adaptor complexes small subunit family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Vesicle

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: Golgi membrane; membrane coat; cytoplasmic vesicle membrane; lysosomal membrane; coated pit; trans-Golgi network membrane; cytosol

Molecular Function: protein transporter activity

Biological Process: viral reproduction; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; regulation of defense response to virus by virus; protein targeting

Disease: Psoriasis 15, Pustular, Susceptibility To