LIM And SH3 Protein 1 Recombinant Protein | LASP1 recombinant protein

Recombinant LIM And SH3 Protein 1 (LASP1)

Cat. Num. AAA2029135

• Gene Names: LASP1; MLN50; Lasp-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: LIM And SH3 Protein 1; Recombinant LIM And SH3 Protein 1 (LASP1); LASP1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MNPNCARCGK IVYPTEKVNC LDKFWHKACF HCETCKMTLN MKNYKGYEKKPYCNAHYPKQ SFTMVADTPE NLRLKQQSEL QSQVRYKEEF EKNKGKGFSVVADTPELQRI KKTQDQISNI KYHEEFEKSR MGPSGGEGME PERRDSQDGSSYRRPLEQQQ PHHIPTSAPV YQQPQQQPVA QSYGGYKEPA APVSIQRSAPGGGGKRYRAV YDYSAADEDE VSFQDGDTIV NVQQIDDGWM YGTVERTGDTGMLPANYVEA I
• Sequence Length: 261

• Applicable Applications for LASP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 33.4kDa
• Accurate Molecular Mass (KD): 38kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Ile261
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 5453710
• NCBI GeneID: 3927
• NCBI Accession #: NP_006139.1
• NCBI GenBank Nucleotide #: NM_006148.3
• Molecular Weight: 23,181 Da
• NCBI Official Full Name: LIM and SH3 domain protein 1 isoform a
• NCBI Official Synonym Full Names: LIM and SH3 protein 1
• NCBI Official Symbol: LASP1
• NCBI Official Synonym Symbols: MLN50; Lasp-1
• NCBI Protein Information: LIM and SH3 domain protein 1
• UniProt Protein Name: LIM and SH3 domain protein 1
• Protein Family: LIM and SH3 domain protein
• UniProt Gene Name: LASP1
• UniProt Synonym Gene Names: MLN50; LASP-1; MLN 50
• UniProt Entry Name: LASP1_HUMAN

NCBI Description

This gene encodes a member of a subfamily of LIM proteins, characterized by a LIM motif and a domain of Src homology region 3, and also a member of the nebulin family of actin-binding proteins. The encoded protein is a cAMP and cGMP dependent signaling protein and binds to the actin cytoskeleton at extensions of the cell membrane. The encoded protein has been linked to metastatic breast cancer, hematopoetic tumors such as B-cell lymphomas, and colorectal cancer. [provided by RefSeq, Oct 2012]

Uniprot Description

Lasp-1: a member of the LIM protein subfamily, characterized by a LIM motif and SH3 domain. Functions as an actin-binding protein, possibly in cytoskeletal organization.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11-q21.3

Cellular Component: cortical actin cytoskeleton; focal adhesion

Molecular Function: actin filament binding; protein binding; SH3/SH2 adaptor activity; zinc ion binding; ion transmembrane transporter activity

Biological Process: positive regulation of signal transduction; ion transport