AOC3 sirna

AOC3 siRNA (Human)

Cat. Num. AAA8220332

• Gene Names: AOC3; HPAO; SSAO; VAP1; VAP-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AOC3; AOC3 siRNA (Human); VAP1; Membrane primary amine oxidase; Copper amine oxidase; HPAO; Semicarbazide-sensitive amine oxidase; SSAO; Vascular adhesion protein 1; VAP-1; AOC3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AOC3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 634

• Applicable Applications for AOC3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AOC3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AOC3 sirna

siRNA to inhibit AOC3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 480306390
• NCBI GeneID: 8639
• NCBI Accession #: NP_001264660.1
• NCBI GenBank Nucleotide #: NM_001277731.1
• UniProt Accession #: Q16853; Q45F94; B2RCI5; K7ESB3; L0L8N9
• Molecular Weight: 24,533 Da
• NCBI Official Full Name: membrane primary amine oxidase isoform 2
• NCBI Official Synonym Full Names: amine oxidase, copper containing 3
• NCBI Official Symbol: AOC3
• NCBI Official Synonym Symbols: HPAO; SSAO; VAP1; VAP-1
• NCBI Protein Information: membrane primary amine oxidase
• UniProt Protein Name: Membrane primary amine oxidase
• Protein Family: Allene oxide cyclase
• UniProt Gene Name: AOC3
• UniProt Synonym Gene Names: VAP1; SSAO; VAP-1
• UniProt Entry Name: AOC3_HUMAN

NCBI Description

This gene encodes a member of the semicarbazide-sensitive amine oxidase family. Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The encoded protein is localized to the cell surface, has adhesive properties as well as monoamine oxidase activity, and may be involved in leukocyte trafficking. Alterations in levels of the encoded protein may be associated with many diseases, including diabetes mellitus. A pseudogene of this gene has been described and is located approximately 9-kb downstream on the same chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]

Uniprot Description

AOC3: Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin- independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis. Belongs to the copper/topaquinone oxidase family.

Protein type: Amino Acid Metabolism - tyrosine; EC 1.4.3.21; Cell adhesion; Membrane protein, integral; Amino Acid Metabolism - glycine, serine and threonine; Other Amino Acids Metabolism - beta-alanine; Oxidoreductase; Amino Acid Metabolism - phenylalanine

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cell surface; microvillus; cytoplasm; plasma membrane; integral to membrane

Molecular Function: amine oxidase activity; protein binding; copper ion binding; protein homodimerization activity; protein heterodimerization activity; cation channel activity; calcium ion binding; quinone binding

Biological Process: response to antibiotic; amine metabolic process; inflammatory response; cell adhesion; cation transport