ALG9 sirna

ALG9 siRNA (Human)

Cat. Num. AAA8214517

• Gene Names: ALG9; CDG1L; DIBD1; LOH11CR1J
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ALG9; ALG9 siRNA (Human); DIBD1; Alpha-1.2-mannosyltransferase ALG9; Asparagine-linked glycosylation protein 9 homolog; Disrupted in bipolar disorder protein 1; Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1.2-mannosyltransferase; Dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1.2-mannosyltransferase; ALG9 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ALG9 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 611

• Applicable Applications for ALG9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ALG9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ALG9 sirna

siRNA to inhibit ALG9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 118026933
• NCBI GeneID: 79796
• NCBI Accession #: NP_001071158.1
• NCBI GenBank Nucleotide #: NM_001077690.1
• UniProt Accession #: Q9H6U8; Q6ZMD5; Q7Z4R4; Q96GS7; Q96PB9; Q9H068
• Molecular Weight: 51,743 Da
• NCBI Official Full Name: alpha-1,2-mannosyltransferase ALG9 isoform b
• NCBI Official Synonym Full Names: ALG9, alpha-1,2-mannosyltransferase
• NCBI Official Symbol: ALG9
• NCBI Official Synonym Symbols: CDG1L; DIBD1; LOH11CR1J
• NCBI Protein Information: alpha-1,2-mannosyltransferase ALG9
• UniProt Protein Name: Alpha-1,2-mannosyltransferase ALG9
• Protein Family: Alpha-1,2-mannosyltransferase
• UniProt Gene Name: ALG9
• UniProt Synonym Gene Names: DIBD1
• UniProt Entry Name: ALG9_HUMAN

NCBI Description

This gene encodes an alpha-1,2-mannosyltransferase enzyme that functions in lipid-linked oligosaccharide assembly. Mutations in this gene result in congenital disorder of glycosylation type Il. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

Uniprot Description

ALG9: Catalyzes the transfer of mannose from Dol-P-Man to lipid-linked oligosaccharides. A chromosomal aberration involving ALG9 is found in a family with bipolar affective disorder. Translocation t(9;11)(p24;q23). However, common variations in ALG9 do not play a major role in predisposition to bipolar affective disorder. Defects in ALG9 are the cause of congenital disorder of glycosylation type 1L (CDG1L). CDGs are a family of severe inherited diseases caused by a defect in protein N- glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the glycosyltransferase 22 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transferase; Membrane protein, multi-pass; EC 2.4.1.261; Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.259

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: mannosyltransferase activity

Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification

Disease: Congenital Disorder Of Glycosylation, Type Il