ALDH4A1 sirna

ALDH4A1 siRNA (Human)

Cat. Num. AAA8220559

• Gene Names: ALDH4A1; P5CD; ALDH4; P5CDh
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ALDH4A1; ALDH4A1 siRNA (Human); ALDH4; P5CDH; Delta-1-pyrroline-5-carboxylate dehydrogenase mitochondrial; P5C dehydrogenase; Aldehyde dehydrogenase family 4 member A1; L-glutamate gamma-semialdehyde dehydrogenase; ALDH4A1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ALDH4A1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 503

• Applicable Applications for ALDH4A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ALDH4A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ALDH4A1 sirna

siRNA to inhibit ALDH4A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 238859541
• NCBI GeneID: 8659
• NCBI Accession #: NP_001154976.1
• NCBI GenBank Nucleotide #: NM_001161504.1
• UniProt Accession #: P30038; Q16882; Q53HU4; Q5JNV6; Q8IZ38; Q96IF0; Q9UDI6; A8K1Q7; B4DGE4; D2D4A3
• Molecular Weight: 56,043 Da
• NCBI Official Full Name: delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial isoform b
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 4 family, member A1
• NCBI Official Symbol: ALDH4A1
• NCBI Official Synonym Symbols: P5CD; ALDH4; P5CDh
• NCBI Protein Information: delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
• UniProt Protein Name: Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
• UniProt Gene Name: ALDH4A1
• UniProt Synonym Gene Names: ALDH4; P5CDH; P5C dehydrogenase
• UniProt Entry Name: AL4A1_HUMAN

NCBI Description

This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

ALDH4A1: Irreversible conversion of delta-1-pyrroline-5- carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes. Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2). HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation. Belongs to the aldehyde dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Oxidoreductase; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 1.2.1.88; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: mitochondrial matrix

Molecular Function: identical protein binding; aldehyde dehydrogenase (NAD) activity; electron carrier activity; 1-pyrroline-5-carboxylate dehydrogenase activity

Biological Process: 4-hydroxyproline catabolic process; proline catabolic process; proline biosynthetic process; glutamate biosynthetic process; proline catabolic process to glutamate; proline metabolic process

Disease: Hyperprolinemia, Type Ii