AKT2 sirna

AKT2 siRNA (Human)

Cat. Num. AAA8210931

• Gene Names: AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AKT2; AKT2 siRNA (Human); RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AKT2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 438

• Applicable Applications for AKT2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AKT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AKT2 sirna

siRNA to inhibit AKT2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4502023
• NCBI GeneID: 208
• NCBI Accession #: NP_001617.1
• NCBI GenBank Nucleotide #: NM_001626.5
• UniProt Accession #: P31751; Q05BV0; Q0VAN0; Q0VAN1; Q68GC0; B2RBD8
• Molecular Weight: 51,083 Da
• NCBI Official Full Name: RAC-beta serine/threonine-protein kinase isoform 1
• NCBI Official Synonym Full Names: v-akt murine thymoma viral oncogene homolog 2
• NCBI Official Symbol: AKT2
• NCBI Official Synonym Symbols: PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
• NCBI Protein Information: RAC-beta serine/threonine-protein kinase
• UniProt Protein Name: RAC-beta serine/threonine-protein kinase
• Protein Family: Potassium channel
• UniProt Gene Name: AKT2
• UniProt Synonym Gene Names: PKB beta
• UniProt Entry Name: AKT2_HUMAN

NCBI Description

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

Akt2: an AGC kinase. Plays critical roles in glucose metabolism and the development or maintenance of proper adipose tissue and islet mass for which other Akt/PKB isoforms are unable to fully compensate. Amplified and overexpressed in human ovarian carcinoma cell lines and amplified in some primary ovarian and pancreatic tumors. Antisense blocks invasiveness in xenografts. Expressed in several insulin-responsive tissues, and one case of Type II diabetes has been associated with a likely LOF point mutation. Mouse mutants have defects in insulin response.

Protein type: Kinase, protein; Oncoprotein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; AKT family

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: nucleoplasm; plasma membrane; cell cortex; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; kinase activity; ATP binding

Biological Process: fat cell differentiation; glycogen biosynthetic process; apoptosis; positive regulation of glycogen biosynthetic process; carbohydrate transport; myelin maintenance in the peripheral nervous system; intracellular protein transport across a membrane; positive regulation of vesicle fusion; glucose metabolic process; protein modification process; signal transduction; regulation of cell migration; protein amino acid phosphorylation; positive regulation of fatty acid beta-oxidation; regulation of translation; positive regulation of glucose import; cellular response to insulin stimulus; insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation

Disease: Hypoinsulinemic Hypoglycemia With Hemihypertrophy; Diabetes Mellitus, Noninsulin-dependent