Western Blot (WB)
((0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat KCC1/SLC12A4 Polyclonal Antibody | anti-SLC12A4 antibody
Goat anti-KCC1/SLC12A4 Antibody
Western Blot: Approx 150kDa band observed in Human Heart and Skeletal Muscle lysates (calculated MW of 121kDa according to NP_005063.1). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the SLC12A transporter family. The encoded protein mediates the coupled movement of potassium and chloride ions across the plasma membrane. This gene is expressed ubiquitously. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2013]
Uniprot Description
KCC1: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia. Isoform 4 has no transport activity. Belongs to the SLC12A transporter family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter; Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: integral to plasma membrane; lysosomal membrane; membrane; plasma membrane
Molecular Function: potassium ion symporter activity; potassium:chloride symporter activity; protein kinase binding
Biological Process: cell volume homeostasis; chloride transport; ion transport; synaptic transmission; transport
Research Articles on SLC12A4
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Product Notes
The SLC12A4 slc12a4 (Catalog #AAA420168) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-KCC1/SLC12A4 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's KCC1/SLC12A4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 150kDa band observed in Human Heart and Skeletal Muscle lysates (calculated MW of 121kDa according to NP_005063.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the SLC12A4 slc12a4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DPSHAPDNFR E. It is sometimes possible for the material contained within the vial of "KCC1/SLC12A4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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