AKR1C3 sirna

AKR1C3 siRNA (Human)

Cat. Num. AAA8220539

• Gene Names: AKR1C3; DD3; DDX; PGFS; HAKRB; HAKRe; HA1753; HSD17B5; hluPGFS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AKR1C3; AKR1C3 siRNA (Human); DDH1; HSD17B5; KIAA0119; PGFS; Aldo-keto reductase family 1 member C3; 17-beta-hydroxysteroid dehydrogenase type 5; 17-beta-HSD 5; 3-alpha-HSD type II. brain; 3-alpha-hydroxysteroid dehydrogenase type 2; 3-alpha-HSD type 2; Chlordecone reductase homolog HAKRb; Dihydrodiol dehydrogenase 3; DD-3; DD3; Dihydrodiol dehydrogenase type I; HA1753; Indanol dehydrogenase; Prostaglandin F synthase; Testosterone 17-beta-dehydrogenase 5; Trans-1.2-dihydrobenzene-1.2-diol dehydrogenase; AKR1C3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AKR1C3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 323

• Applicable Applications for AKR1C3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AKR1C3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AKR1C3 sirna

siRNA to inhibit AKR1C3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 359806990
• NCBI GeneID: 8644
• NCBI Accession #: NP_001240837.1
• NCBI GenBank Nucleotide #: NM_001253908.1
• UniProt Accession #: P42330; Q5T2L1; Q96DJ1; Q96KI8; Q99530; Q9UCX1; Q9UII3; Q9UKL9; A8K2V0; B4DL37
• Molecular Weight: 23,364 Da
• NCBI Official Full Name: aldo-keto reductase family 1 member C3 isoform 2
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member C3
• NCBI Official Symbol: AKR1C3
• NCBI Official Synonym Symbols: DD3; DDX; PGFS; HAKRB; HAKRe; HA1753; HSD17B5; hluPGFS
• NCBI Protein Information: aldo-keto reductase family 1 member C3
• UniProt Protein Name: Aldo-keto reductase family 1 member C3
• Protein Family: Aldo-keto reductase family
• UniProt Gene Name: AKR1C3
• UniProt Synonym Gene Names: DDH1; HSD17B5; KIAA0119; PGFS; 17-beta-HSD 5; 3-alpha-HSD type 2; DD-3; DD3; PGFS
• UniProt Entry Name: AK1C3_HUMAN

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

AKR1C3: Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta- PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone. Belongs to the aldo/keto reductase family.

Protein type: Oxidoreductase; EC 1.1.1.149; Lipid Metabolism - arachidonic acid; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: cytoplasm; intracellular; cytosol; nucleus

Molecular Function: 15-hydroxyprostaglandin-D dehydrogenase (NADP+) activity; delta4-3-oxosteroid 5beta-reductase activity; aldo-keto reductase activity; prostaglandin-F synthase activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity; 3(or 17)-alpha-hydroxysteroid dehydrogenase activity; phenanthrene 9,10-monooxygenase activity; retinol dehydrogenase activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; ketosteroid monooxygenase activity; testosterone 17-beta-dehydrogenase (NADP+) activity; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; ketoreductase activity; indanol dehydrogenase activity; aldehyde reductase activity; geranylgeranyl reductase activity; retinal dehydrogenase activity

Biological Process: steroid metabolic process; phototransduction, visible light; retinal metabolic process; cyclooxygenase pathway; male gonad development; progesterone metabolic process; cellular response to starvation; prostaglandin metabolic process; keratinocyte differentiation; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; protein import into nucleus, translocation; positive regulation of cell proliferation; arachidonic acid metabolic process; farnesol catabolic process; multicellular organismal macromolecule metabolic process; retinoid metabolic process; response to nutrient; regulation of retinoic acid receptor signaling pathway