AIFM2 sirna

AIFM2 siRNA (Human)

Cat. Num. AAA8229607

• Gene Names: AIFM2; AMID; PRG3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AIFM2; AIFM2 siRNA (Human); AMID; PRG3; Apoptosis-inducing factor 2; Apoptosis-inducing factor homologous mitochondrion-associated inducer of death; Apoptosis-inducing factor-like mitochondrion-associated inducer of death; p53-responsive gene 3 protein; AIFM2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AIFM2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 373

• Applicable Applications for AIFM2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AIFM2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AIFM2 sirna

siRNA to inhibit AIFM2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 311082415
• NCBI GeneID: 84883
• NCBI Accession #: NP_001185625.1
• NCBI GenBank Nucleotide #: NM_001198696.1
• UniProt Accession #: Q9BRQ8; Q63Z39; B3KXI0
• Molecular Weight: 36,520 Da
• NCBI Official Full Name: apoptosis-inducing factor 2
• NCBI Official Synonym Full Names: apoptosis-inducing factor, mitochondrion-associated, 2
• NCBI Official Symbol: AIFM2
• NCBI Official Synonym Symbols: AMID; PRG3
• NCBI Protein Information: apoptosis-inducing factor 2
• UniProt Protein Name: Apoptosis-inducing factor 2
• Protein Family: Apoptosis-inducing factor
• UniProt Gene Name: AIFM2
• UniProt Entry Name: AIFM2_HUMAN

NCBI Description

This gene encodes a flavoprotein oxidoreductase that binds single stranded DNA and is thought to contribute to apoptosis in the presence of bacterial and viral DNA. The expression of this gene is also found to be induced by tumor suppressor protein p53 in colon cancer cells. [provided by RefSeq, Nov 2010]

Uniprot Description

AIFM2: Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Binds to DNA in a sequence-independent manner. May contribute to genotoxin-induced growth arrest. Belongs to the FAD-dependent oxidoreductase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Membrane protein, integral; Apoptosis; EC 1.-.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: extracellular space; mitochondrial outer membrane; mitochondrion; cytoplasm; integral to membrane; lipid particle; cytosol

Molecular Function: DNA binding; FAD binding; NADH dehydrogenase (ubiquinone) activity; electron-transferring-flavoprotein dehydrogenase activity

Biological Process: apoptotic mitochondrial changes; positive regulation of apoptosis