AGPS sirna

AGPS siRNA (Human)

Cat. Num. AAA8214931

• Gene Names: AGPS; ADAS; ADPS; ADAP-S; ADHAPS; ALDHPSY
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AGPS; AGPS siRNA (Human); Alkyldihydroxyacetonephosphate synthase peroxisomal; Alkyl-DHAP synthase; Aging-associated gene 5 protein; Alkylglycerone-phosphate synthase; AGPS sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AGPS siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 658

• Applicable Applications for AGPS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AGPS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AGPS sirna

siRNA to inhibit AGPS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4501993
• NCBI GeneID: 8540
• NCBI Accession #: NP_003650.1
• NCBI GenBank Nucleotide #: NM_003659.3
• UniProt Accession #: O00116; Q2TU35; A5D8U9
• Molecular Weight: 72,912 Da
• NCBI Official Full Name: alkyldihydroxyacetonephosphate synthase, peroxisomal
• NCBI Official Synonym Full Names: alkylglycerone phosphate synthase
• NCBI Official Symbol: AGPS
• NCBI Official Synonym Symbols: ADAS; ADPS; ADAP-S; ADHAPS; ALDHPSY
• NCBI Protein Information: alkyldihydroxyacetonephosphate synthase, peroxisomal
• UniProt Protein Name: Alkyldihydroxyacetonephosphate synthase, peroxisomal
• Protein Family: Alkyldihydroxyacetonephosphate synthase
• UniProt Gene Name: AGPS
• UniProt Synonym Gene Names: Alkyl-DHAP synthase
• UniProt Entry Name: ADAS_HUMAN

NCBI Description

This gene is a member of the FAD-binding oxidoreductase/transferase type 4 family. It encodes a protein that catalyzes the second step of ether lipid biosynthesis in which acyl-dihydroxyacetonephosphate (DHAP) is converted to alkyl-DHAP by the addition of a long chain alcohol and the removal of a long-chain acid anion. The protein is localized to the inner aspect of the peroxisomal membrane and requires FAD as a cofactor. Mutations in this gene have been associated with rhizomelic chondrodysplasia punctata, type 3 and Zellweger syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

AGPS: Defects in AGPS are the cause of rhizomelic chondrodysplasia punctata type 3 (RCDP3). RCDP3 is characterized by rhizomelic shortening of femur and humerus, vertebral disorders, cataract, cutaneous lesions and severe mental retardation. Belongs to the FAD-binding oxidoreductase/transferase type 4 family.

Protein type: Transferase; EC 2.5.1.26; Lipid Metabolism - ether lipid; Oxidoreductase; Mitochondrial

Chromosomal Location of Human Ortholog: 2q31.2

Cellular Component: peroxisomal membrane; peroxisomal matrix; membrane; mitochondrion; intracellular membrane-bound organelle; nucleolus; peroxisome

Molecular Function: UDP-N-acetylmuramate dehydrogenase activity; alkylglycerone-phosphate synthase activity

Biological Process: lipid biosynthetic process; cellular lipid metabolic process; ether lipid biosynthetic process

Disease: Rhizomelic Chondrodysplasia Punctata, Type 3