AGPAT6 sirna

AGPAT6 siRNA (Human)

Cat. Num. AAA8210188

• Gene Names: GPAT4; AGPAT6; LPAATZ; TSARG7; 1-AGPAT 6; LPAAT-zeta
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AGPAT6; AGPAT6 siRNA (Human); GPAT4; Glycerol-3-phosphate acyltransferase 4; 1-acylglycerol-3-phosphate O-acyltransferase 6; 1-AGP acyltransferase 6; 1-AGPAT 6; Acyl-CoA:glycerol-3-phosphate acyltransferase 4; Lysophosphatidic acid acyltransferase zeta; LPAAT-zeta; AGPAT6 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AGPAT6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 456

• Applicable Applications for AGPAT6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AGPAT6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AGPAT6 sirna

siRNA to inhibit AGPAT6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 30520329
• NCBI GeneID: 137964
• NCBI Accession #: NP_848934.1
• NCBI GenBank Nucleotide #: NM_178819.3
• UniProt Accession #: Q86UL3; Q86V89
• Molecular Weight: 52,071 Da
• NCBI Official Full Name: glycerol-3-phosphate acyltransferase 4
• NCBI Official Synonym Full Names: glycerol-3-phosphate acyltransferase 4
• NCBI Official Symbol: GPAT4
• NCBI Official Synonym Symbols: AGPAT6; LPAATZ; TSARG7; 1-AGPAT 6; LPAAT-zeta
• NCBI Protein Information: glycerol-3-phosphate acyltransferase 4
• UniProt Protein Name: Glycerol-3-phosphate acyltransferase 4
• UniProt Gene Name: AGPAT6
• UniProt Synonym Gene Names: GPAT4; GPAT4; 1-AGP acyltransferase 6; 1-AGPAT 6; LPAAT-zeta
• UniProt Entry Name: GPAT4_HUMAN

NCBI Description

Lysophosphatidic acid acyltransferases (EC 2.3.1.51) catalyze the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA). LPA and PA are involved in signal transduction and lipid biosynthesis.[supplied by OMIM, Apr 2004]

Uniprot Description

AGPAT6: Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Active against both saturated and unsaturated long- chain fatty acyl-CoAs. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Protein type: Lipid Metabolism - glycerophospholipid; Endoplasmic reticulum; Lipid Metabolism - ether lipid; EC 2.3.1.15; Membrane protein, multi-pass; Membrane protein, integral; Lipid Metabolism - glycerolipid; Transferase

Chromosomal Location of Human Ortholog: 8p11.21

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: glycerol-3-phosphate O-acyltransferase activity; 1-acylglycerol-3-phosphate O-acyltransferase activity

Biological Process: lactation; acyl-CoA metabolic process; lipid biosynthetic process; glycerophospholipid biosynthetic process; glandular epithelial cell maturation; regulation of multicellular organism growth; triacylglycerol biosynthetic process; cellular lipid metabolic process; fatty acid metabolic process; phospholipid metabolic process; phosphatidylcholine biosynthetic process; phosphatidic acid biosynthetic process; diacylglycerol metabolic process; CDP-diacylglycerol biosynthetic process