Rabbit anti-Human EphA7 Polyclonal Antibody | anti-EphA7 antibody

EphA7, CT (EPHA7, EHK3, HEK11, Ephrin type-A receptor 7, EPH homology kinase 3, EPH-like kinase 11) (PE)

Cat. Num. AAA6296017

• Gene Names: EPHA7; EHK3; HEK11
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: EphA7; Polyclonal Antibody; CT (EPHA7; EHK3; HEK11; Ephrin type-A receptor 7; EPH homology kinase 3; EPH-like kinase 11) (PE); EPHA7; EPH-like kinase 11; anti-EphA7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-EphA7 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: EphA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 904~933 amino acids from the C-terminal region of human EphA7.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-EphA7 Pab in NCI-H460 cell lysate. EphA7 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB)

(Western blot analysis of EPHA7 (arrow) using EphA7 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA7 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-EphA7 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Product Categories/Family for anti-EphA7 antibody

Antibodies; Protein Kinases

References

Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001). Xu, Q., et al., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 355(1399):993-1002 (2000). Holder, N., et al., Development 126(10):2033-2044 (1999). Zhou, R., Pharmacol. Ther. 77(3):151-181 (1998). Fox, G.M., et al., Oncogene 10(5):897-905 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 4758282
• NCBI GeneID: 2045
• NCBI Accession #: NP_004431.1
• NCBI GenBank Nucleotide #: NM_004440.3
• UniProt Accession #: Q15375; Q59G40; Q5VTU0; Q8N368; Q9H124; A0AUX7; B2R8W1; B7ZLJ9; B7ZLK0
• Molecular Weight: 112,097 Da
• NCBI Official Full Name: ephrin type-A receptor 7
• NCBI Official Synonym Full Names: EPH receptor A7
• NCBI Official Symbol: EPHA7
• NCBI Official Synonym Symbols: EHK3; HEK11
• NCBI Protein Information: ephrin type-A receptor 7; EK11; EHK-3; EPH-like kinase 11; EPH homology kinase 3; Eph homology kinase-3; receptor protein-tyrosine kinase HEK11; tyrosine-protein kinase receptor EHK-3
• UniProt Protein Name: Ephrin type-A receptor 7
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA7
• UniProt Synonym Gene Names: EHK3; HEK11; EHK-3; EK11; hEK11
• UniProt Entry Name: EPHA7_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

Uniprot Description

EphA7: a widely-expressed receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A2, -A3, -A4 and -A5. Contains 1 sterile alpha motif (SAM) and two fibronectin type III domains.

Protein type: Protein kinase, TK; Membrane protein, integral; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Eph family

Chromosomal Location of Human Ortholog: 6q16.1

Cellular Component: postsynaptic membrane; cell soma; integral to plasma membrane; dendrite; plasma membrane; neuromuscular junction

Molecular Function: protein binding; ephrin receptor binding; chemorepellent activity; protein-tyrosine kinase activity; GPI-linked ephrin receptor activity; axon guidance receptor activity; ATP binding

Biological Process: axon guidance; regulation of cell-cell adhesion; peptidyl-tyrosine phosphorylation; branching morphogenesis of a nerve; regulation of protein amino acid autophosphorylation; positive regulation of neuron apoptosis; ephrin receptor signaling pathway; negative chemotaxis; brain development; regulation of caspase activity; phosphorylation; regulation of peptidyl-tyrosine phosphorylation; retinal ganglion cell axon guidance