AGBL1 sirna

AGBL1 siRNA (Human)

Cat. Num. AAA8211448

• Gene Names: AGBL1; CCP4; FECD8
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AGBL1; AGBL1 siRNA (Human); CCP4; Cytosolic carboxypeptidase 4; ATP/GTP-binding protein-like 1; AGBL1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AGBL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1066

• Applicable Applications for AGBL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AGBL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AGBL1 sirna

siRNA to inhibit AGBL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 120444924
• NCBI GeneID: 123624
• NCBI Accession #: NP_689549.2
• NCBI GenBank Nucleotide #: NM_152336.2
• UniProt Accession #: Q96MI9; A1A4X5; A6NJH6; C9JHL5
• Molecular Weight: 90,789 Da
• NCBI Official Full Name: cytosolic carboxypeptidase 4
• NCBI Official Synonym Full Names: ATP/GTP binding protein-like 1
• NCBI Official Symbol: AGBL1
• NCBI Official Synonym Symbols: CCP4; FECD8
• NCBI Protein Information: cytosolic carboxypeptidase 4
• UniProt Protein Name: Cytosolic carboxypeptidase 4
• Protein Family: Cytosolic carboxypeptidase
• UniProt Gene Name: AGBL1
• UniProt Synonym Gene Names: CCP4
• UniProt Entry Name: CBPC4_HUMAN

NCBI Description

Polyglutamylation is a reversible posttranslational modification catalyzed by polyglutamylases that results in the addition of glutamate side chains on the modified protein. This gene encodes a glutamate decarboxylase that catalyzes the deglutamylation of polyglutamylated proteins. Mutations in this gene result in dominant late-onset Fuchs corneal dystrophy. [provided by RefSeq, Nov 2013]

Uniprot Description

AGBL1: Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5. Belongs to the peptidase M14 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.17.-; Protease

Chromosomal Location of Human Ortholog: 15q25.3

Cellular Component: cytosol

Molecular Function: tubulin binding; zinc ion binding; metallocarboxypeptidase activity

Biological Process: proteolysis

Disease: Corneal Dystrophy, Fuchs Endothelial, 8