ADK sirna

ADK siRNA (Human)

Cat. Num. AAA8237215

• Gene Names: ADK; AK
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADK; ADK siRNA (Human); Adenosine kinase; AK; Adenosine 5'-phosphotransferase; ADK sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ADK siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 327

• Applicable Applications for ADK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADK sirna

siRNA to inhibit ADK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 32484973
• NCBI GeneID: 132
• NCBI Accession #: NP_001114.2
• NCBI GenBank Nucleotide #: NM_001123.3
• UniProt Accession #: P55263; O00741; O00742; Q16710; Q5JQ10; Q5JQ11; Q9BTN2; B7Z783; B7Z800
• Molecular Weight: 36,580 Da
• NCBI Official Full Name: adenosine kinase isoform a
• NCBI Official Synonym Full Names: adenosine kinase
• NCBI Official Symbol: ADK
• NCBI Official Synonym Symbols: AK
• NCBI Protein Information: adenosine kinase
• UniProt Protein Name: Adenosine kinase
• UniProt Gene Name: ADK
• UniProt Synonym Gene Names: AK
• UniProt Entry Name: ADK_HUMAN

NCBI Description

This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

ADK: ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. Defects in ADK are the cause of hypermethioninemia due to adenosine kinase deficiency (HMAKD). A metabolic disorder characterized by global developmental delay, early-onset seizures, mild dysmorphic features, and characteristic biochemical anomalies, including persistent hypermethioninemia with increased levels of S-adenosylmethionine and S-adenosylhomocysteine. Homocysteine levels are typically normal. Belongs to the carbohydrate kinase PfkB family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, other; EC 2.7.1.20; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 10q22|10q11-q24

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: metal ion binding; adenosine kinase activity; phosphotransferase activity, alcohol group as acceptor; ATP binding

Biological Process: nucleobase, nucleoside and nucleotide metabolic process; dATP biosynthetic process; ribonucleoside monophosphate biosynthetic process; positive regulation of T cell proliferation; circadian regulation of gene expression; adenosine metabolic process; phosphorylation; purine salvage; purine base metabolic process

Disease: Hypermethioninemia Due To Adenosine Kinase Deficiency