ADH1A sirna

ADH1A siRNA (Human)

Cat. Num. AAA8219603

• Gene Names: ADH1A; ADH1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADH1A; ADH1A siRNA (Human); ADH1; Alcohol dehydrogenase 1A; Alcohol dehydrogenase subunit alpha; ADH1A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ADH1A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 375

• Applicable Applications for ADH1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADH1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADH1A sirna

siRNA to inhibit ADH1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4501929
• NCBI GeneID: 124
• NCBI Accession #: NP_000658.1
• NCBI GenBank Nucleotide #: NM_000667.3
• UniProt Accession #: P07327; Q17R68; A8K3E3
• Molecular Weight: 39,859 Da
• NCBI Official Full Name: alcohol dehydrogenase 1A
• NCBI Official Synonym Full Names: alcohol dehydrogenase 1A (class I), alpha polypeptide
• NCBI Official Symbol: ADH1A
• NCBI Official Synonym Symbols: ADH1
• NCBI Protein Information: alcohol dehydrogenase 1A
• UniProt Protein Name: Alcohol dehydrogenase 1A
• UniProt Gene Name: ADH1A
• UniProt Synonym Gene Names: ADH1
• UniProt Entry Name: ADH1A_HUMAN

NCBI Description

This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010]

Uniprot Description

ADH1A: a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010]

Protein type: Cofactor and Vitamin Metabolism - retinol; EC 1.1.1.1; Transcription regulation; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lipid Metabolism - fatty acid; Xenobiotic Metabolism - metabolism by cytochrome P450; Amino Acid Metabolism - tyrosine; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytosol

Molecular Function: protein binding; zinc ion binding; alcohol dehydrogenase activity; alcohol dehydrogenase activity, zinc-dependent

Biological Process: xenobiotic metabolic process; alcohol metabolic process; drug metabolic process; ethanol oxidation