ADAR sirna

ADAR siRNA (Human)

Cat. Num. AAA8218283

• Gene Names: ADAR; DSH; AGS6; G1P1; IFI4; P136; ADAR1; DRADA; DSRAD; IFI-4; K88DSRBP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADAR; ADAR siRNA (Human); ADAR1; DSRAD; G1P1; IFI4; Double-stranded RNA-specific adenosine deaminase; DRADA; 136 kDa double-stranded RNA-binding protein; p136; Interferon-inducible protein 4; IFI-4; K88DSRBP; ADAR sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ADAR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 931

• Applicable Applications for ADAR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADAR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADAR sirna

siRNA to inhibit ADAR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 70167113
• NCBI GeneID: 103
• NCBI Accession #: NP_001020278.1
• NCBI GenBank Nucleotide #: NM_001025107.2
• UniProt Accession #: P55265; O15223; O43859; O43860; Q9BYM3; Q9BYM4; B1AQQ9; B1AQR0; D3DV76
• Molecular Weight: 103,642 Da
• NCBI Official Full Name: double-stranded RNA-specific adenosine deaminase isoform d
• NCBI Official Synonym Full Names: adenosine deaminase, RNA-specific
• NCBI Official Symbol: ADAR
• NCBI Official Synonym Symbols: DSH; AGS6; G1P1; IFI4; P136; ADAR1; DRADA; DSRAD; IFI-4; K88DSRBP
• NCBI Protein Information: double-stranded RNA-specific adenosine deaminase
• UniProt Protein Name: Double-stranded RNA-specific adenosine deaminase
• Protein Family: Double-stranded RNA-specific editase
• UniProt Gene Name: ADAR
• UniProt Synonym Gene Names: ADAR1; DSRAD; G1P1; IFI4; DRADA; p136; IFI-4
• UniProt Entry Name: DSRAD_HUMAN

NCBI Description

This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]

Uniprot Description

ADAR: Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site- selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression. Isoform 1 is induced by interferon alpha. Isoform 5 is constitutively expressed. Homodimer. Isoform 1 interacts with ILF2/NF45 and ILF3/NF90. 5 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 3.5.4.37; RNA processing; Nucleolus; Hydrolase; RNA-binding

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus

Molecular Function: double-stranded RNA adenosine deaminase activity; protein binding; DNA binding; metal ion binding

Biological Process: positive regulation of viral genome replication; base conversion or substitution editing; in utero embryonic development; response to virus; cytokine and chemokine mediated signaling pathway; miRNA-mediated gene silencing, miRNA loading onto RISC; somatic diversification of immune receptors via somatic mutation; adenosine to inosine editing; osteoblast differentiation; negative regulation of viral genome replication; pre-microRNA processing; protein import into nucleus; mRNA modification; erythrocyte differentiation; innate immune response; hemopoietic progenitor cell differentiation; gene expression; protein export from nucleus; mRNA processing; defense response to virus; negative regulation of apoptosis

Disease: Dyschromatosis Symmetrica Hereditaria; Aicardi-goutieres Syndrome 6