Human ADAR (Double-stranded RNA-specific adenosine deaminase) ELISA Kit | ADAR elisa kit

Human ADAR (Double-stranded RNA-specific adenosine deaminase) ELISA Kit

Cat. Num. AAA7612656

• Gene Names: ADAR; DSH; AGS6; G1P1; IFI4; P136; ADAR1; DRADA; DSRAD; IFI-4; K88DSRBP
• Reactivity: Human
• Synonyms: ADAR (Double-stranded RNA-specific adenosine deaminase); Human ADAR (Double-stranded RNA-specific adenosine deaminase) ELISA Kit; Double-stranded RNA-specific adenosine deaminase; DRADA; 136 kDa double-stranded RNA-binding protein; p136; Interferon-inducible protein 4; IFI-4; K88DSRBP; ADAR1; DSRAD; G1P1; IFI4; ADAR; ADAR elisa kit

• Reactivity: Human

• Assay Type: Sandwich ELISA, Double Antibody
• Samples: serum, plasma, tissue homogenates and other biological fluids.
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Precision: Intra-Assay: CV<8%; Inter-Assay: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 70167113
• NCBI GeneID: 103
• NCBI Accession #: NP_001020278.1
• NCBI GenBank Nucleotide #: NM_001025107.2
• UniProt Accession #: P55265; O15223; O43859; O43860; Q9BYM3; Q9BYM4; B1AQQ9; B1AQR0; D3DV76
• Molecular Weight: 136,066 Da
• NCBI Official Full Name: double-stranded RNA-specific adenosine deaminase isoform d
• NCBI Official Synonym Full Names: adenosine deaminase, RNA-specific
• NCBI Official Symbol: ADAR
• NCBI Official Synonym Symbols: DSH; AGS6; G1P1; IFI4; P136; ADAR1; DRADA; DSRAD; IFI-4; K88DSRBP
• NCBI Protein Information: double-stranded RNA-specific adenosine deaminase; dsRNA adenosine deaminase; interferon-induced protein 4; interferon-inducible protein 4; adenosine deaminase acting on RNA 1-A; 136 kDa double-stranded RNA-binding protein
• UniProt Protein Name: Double-stranded RNA-specific adenosine deaminase
• Protein Family: Double-stranded RNA-specific editase
• UniProt Gene Name: ADAR
• UniProt Synonym Gene Names: ADAR1; DSRAD; G1P1; IFI4; DRADA; p136; IFI-4
• UniProt Entry Name: DSRAD_HUMAN

NCBI Description

This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]

Uniprot Description

ADAR: Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site- selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression. Isoform 1 is induced by interferon alpha. Isoform 5 is constitutively expressed. Homodimer. Isoform 1 interacts with ILF2/NF45 and ILF3/NF90. 5 isoforms of the human protein are produced by alternative promoter.

Protein type: RNA-binding; RNA processing; Nucleolus; Hydrolase; EC 3.5.4.37

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus

Molecular Function: double-stranded RNA adenosine deaminase activity; protein binding; DNA binding; metal ion binding

Biological Process: positive regulation of viral genome replication; base conversion or substitution editing; in utero embryonic development; response to virus; cytokine and chemokine mediated signaling pathway; miRNA-mediated gene silencing, miRNA loading onto RISC; somatic diversification of immune receptors via somatic mutation; adenosine to inosine editing; osteoblast differentiation; negative regulation of viral genome replication; protein import into nucleus; pre-microRNA processing; mRNA modification; innate immune response; erythrocyte differentiation; gene expression; hemopoietic progenitor cell differentiation; protein export from nucleus; mRNA processing; defense response to virus; negative regulation of apoptosis

Disease: Dyschromatosis Symmetrica Hereditaria; Aicardi-goutieres Syndrome 6