ACO1 sirna

ACO1 siRNA (Human)

Cat. Num. AAA8237632

• Gene Names: ACO1; IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ACO1; ACO1 siRNA (Human); IREB1; Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1; ACO1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ACO1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 889

• Applicable Applications for ACO1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ACO1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ACO1 sirna

siRNA to inhibit ACO1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 507834063
• NCBI GeneID: 48
• NCBI Accession #: NP_001265281.1
• NCBI GenBank Nucleotide #: NM_001278352.1
• UniProt Accession #: P21399; Q14652; Q5VZA7; D3DRK7
• Molecular Weight: 98,399 Da
• NCBI Official Full Name: cytoplasmic aconitate hydratase
• NCBI Official Synonym Full Names: aconitase 1, soluble
• NCBI Official Symbol: ACO1
• NCBI Official Synonym Symbols: IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1
• NCBI Protein Information: cytoplasmic aconitate hydratase
• UniProt Protein Name: Cytoplasmic aconitate hydratase
• Protein Family: Aconitate hydratase
• UniProt Gene Name: ACO1
• UniProt Synonym Gene Names: IREB1; Aconitase; IRP1; IRE-BP 1
• UniProt Entry Name: ACOC_HUMAN

NCBI Description

The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]

Uniprot Description

IREB1: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Belongs to the aconitase/IPM isomerase family.

Protein type: EC 4.2.1.3; RNA-binding; Carbohydrate Metabolism - citrate (TCA) cycle; Endoplasmic reticulum; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Translation; Lyase

Chromosomal Location of Human Ortholog: 9p21.1

Cellular Component: Golgi apparatus; mitochondrion; endoplasmic reticulum; cytoplasm; cytosol

Molecular Function: protein binding; iron-responsive element binding; RNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; aconitate hydratase activity

Biological Process: regulation of translation; cellular iron ion homeostasis; tricarboxylic acid cycle; citrate metabolic process; response to iron(II) ion; post-embryonic development; intestinal absorption