ACAP1 sirna

ACAP1 siRNA (Human)

Cat. Num. AAA8200608

• Gene Names: ACAP1; CENTB1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ACAP1; ACAP1 siRNA (Human); CENTB1; KIAA0050; Arf-GAP with coiled-coil. ANK repeat and PH domain-containing protein 1; Centaurin-beta-1; Cnt-b1; ACAP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ACAP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 740

• Applicable Applications for ACAP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ACAP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ACAP1 sirna

siRNA to inhibit ACAP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7661880
• NCBI GeneID: 9744
• NCBI Accession #: NP_055531.1
• NCBI GenBank Nucleotide #: NM_014716.3
• UniProt Accession #: Q15027; Q53XN9
• Molecular Weight: 81,536 Da
• NCBI Official Full Name: arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
• NCBI Official Synonym Full Names: ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
• NCBI Official Symbol: ACAP1
• NCBI Official Synonym Symbols: CENTB1
• NCBI Protein Information: arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
• UniProt Protein Name: Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
• Protein Family: Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein
• UniProt Gene Name: ACAP1
• UniProt Synonym Gene Names: CENTB1; KIAA0050; Cnt-b1
• UniProt Entry Name: ACAP1_HUMAN

Uniprot Description

CENTB1: a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins (GAPs). A GAP for ARF1 and ARF5. Two alternatively-spliced isoforms have been described. Isoform 1 is brain-specific, and isoform 2 is ubiquitously expressed, with highest levels in brain and heart. Isoform 1 participates to the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. Regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. The PH domains of isoforms 1 and 2 bind phospholipids, but they differentially regulate subcellular location. The PH domain of isoform 1 directs the protein to the nucleus, but the PH domain of isoform 2 directs it to the cytosol.

Protein type: GAPs, ARF; Motility/polarity/chemotaxis; GAPs

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: membrane; recycling endosome membrane

Molecular Function: zinc ion binding

Biological Process: protein transport; positive regulation of GTPase activity