ABCB6 sirna

ABCB6 siRNA (Human)

Cat. Num. AAA8226576

• Gene Names: ABCB6; ABC; LAN; PRP; DUH3; umat; ABC14; MTABC3; MCOPCB7
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ABCB6; ABCB6 siRNA (Human); MTABC3; PRP; UMAT; ATP-binding cassette sub-family B member 6 mitochondrial; Mitochondrial ABC transporter 3; Mt-ABC transporter 3; P-glycoprotein-related protein; Ubiquitously-expressed mammalian ABC half transporter; ABCB6 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ABCB6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 796

• Applicable Applications for ABCB6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ABCB6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ABCB6 sirna

siRNA to inhibit ABCB6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9955963
• NCBI GeneID: 10058
• NCBI Accession #: NP_005680.1
• NCBI GenBank Nucleotide #: NM_005689.2
• UniProt Accession #: Q9NP58; O75542; Q49A66; Q59GQ5; Q6ZME6; Q96ME8; Q9HAQ6; Q9HAQ7
• Molecular Weight: 88,600 Da
• NCBI Official Full Name: ATP-binding cassette sub-family B member 6, mitochondrial
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 6 (Langereis blood group)
• NCBI Official Symbol: ABCB6
• NCBI Official Synonym Symbols: ABC; LAN; PRP; DUH3; umat; ABC14; MTABC3; MCOPCB7
• NCBI Protein Information: ATP-binding cassette sub-family B member 6, mitochondrial
• UniProt Protein Name: ATP-binding cassette sub-family B member 6, mitochondrial
• Protein Family: ABC transporter B family
• UniProt Gene Name: ABCB6
• UniProt Synonym Gene Names: MTABC3; PRP; UMAT; Mt-ABC transporter 3
• UniProt Entry Name: ABCB6_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This half-transporter likely plays a role in mitochondrial function. Localized to 2q26, this gene is considered a candidate gene for lethal neonatal metabolic syndrome, a disorder of mitochondrial function. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCB6: Binds heme and porphyrins and functions in their ATP- dependent uptake into the mitochondria. Plays a crucial role in heme synthesis. Defects in ABCB6 are the cause of microphthalmia, isolated, with coloboma, type 7 (MCOPCB7). A disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ABC family; Membrane protein, integral; Hydrolase; Membrane protein, multi-pass; Transporter; Mitochondrial

Chromosomal Location of Human Ortholog: 2q36

Cellular Component: mitochondrial envelope; Golgi apparatus; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; plasma membrane; ATP-binding cassette (ABC) transporter complex; endosome; integral to mitochondrial outer membrane

Molecular Function: efflux transmembrane transporter activity; heme-transporting ATPase activity; heme transporter activity; heme binding; ATP binding

Biological Process: skin development; heme transport; transport; cellular iron ion homeostasis; brain development; transmembrane transport; porphyrin biosynthetic process

Disease: Dyschromatosis Universalis Hereditaria 3; Blood Group, Langereis System; Microphthalmia, Isolated, With Coloboma 7