MED15 blocking peptide

MED15 Blocking Peptide

Cat. Num. AAA823811

• Gene Names: MED15; TIG1; CAG7A; CTG7A; PCQAP; TIG-1; TNRC7; ARC105
• Reactivity: Human, Mouse, Rat, Zebrafish
• Applications: Blocking (BL)
• Purity: >85%
• Synonyms: MED15; MED15 Blocking Peptide; ARC105; CTG7A; PCQAP; TIG1; TNRC7; Mediator of RNA polymerase II transcription subunit 15; Activator-recruited cofactor 105 kDa component; CTG repeat protein 7a; Mediator complex subunit 15; Positive cofactor 2 glutamine/Q-rich-associated protein; PC2 glutamine/Q-rich-associated protein; TPA-inducible gene 1 protein; TIG-1; Trinucleotide repeat-containing gene 7 protein; MED15 blocking peptide

• Host: Synthetic
• Reactivity: Human, Mouse, Rat, Zebrafish
• Purity/Purification: >85%
• Form/Format: Lyophilized powder
• Sequence Length: 788

• Applicable Applications for MED15 blocking peptide: Blocking (BL)
• Application Notes: Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
• Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MED15 blocking peptide

The peptide is used to block Anti-MED15 Antibody reactivity.

NCBI and Uniprot Product Information

• NCBI GI #: 51477700
• NCBI GeneID: 51586
• NCBI Accession #: NP_001003891.1
• NCBI GenBank Nucleotide #: NM_001003891.2
• UniProt Accession #: Q96RN5; O15413; Q6IC31; Q8NF16; Q96CT0; Q96IH7; Q9P1T3; D3DX31; D3DX32
• Molecular Weight: 75,927 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 15 isoform a
• NCBI Official Synonym Full Names: mediator complex subunit 15
• NCBI Official Symbol: MED15
• NCBI Official Synonym Symbols: TIG1; CAG7A; CTG7A; PCQAP; TIG-1; TNRC7; ARC105
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 15; TPA inducible gene-1; CTG repeat protein 7a; TPA inducible protein; TPA-inducible gene 1 protein; trinucleotide repeat containing 7; activator-recruited cofactor, 105-kD; PC2-glutamine-rich-associated protein; PC2 glutamine/Q-rich-associated protein; activator-recruited cofactor 105 kDa component; trinucleotide repeat-containing gene 7 protein; positive cofactor 2 glutamine/Q-rich-associated protein; positive cofactor 2, glutamine/Q-rich-associated protein; PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 15
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED15
• UniProt Synonym Gene Names: ARC105; CTG7A; PCQAP; TIG1; TNRC7; ARC105; PC2 glutamine/Q-rich-associated protein; TIG-1
• UniProt Entry Name: MED15_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Uniprot Description

PCQAP: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway. Belongs to the Mediator complex subunit 15 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 22q11.2

Cellular Component: nucleoplasm; membrane; cytoplasm; Srb-mediator complex; nucleus

Molecular Function: protein binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; stem cell maintenance; gene expression